Brain-Derived Neurotrophic Factor (BDNF)

Brain Derived Neurotrophic Factor and the “Attività plastic dei neuroni”, the Neuronal Plasticity as Defined by Ernesto Lugaro (1870-1940).- Usage of Bacterial Artificial Chromosomes for Studying BDNF Gene Regulation in Primary Cultures of Cortical Neurons and Astrocytes.- Detecting Single and Multiple BDNF Transcripts In Situ in Neuronal Cultures and Brain Sections.- Studying BDNF/TrkB Signaling: Transcriptome Analysis from a Limited Number of Purified Adult or Aged Murine Brain Neurons.- Studying BDNF/TrkB Signaling: High-Throughput Microfluidic Gene Expression Analysis from rare of Limited Samples of Adult and Aged Central Neurons.- Detecting BDNF Protein Forms by ELISA, Western Blot, and Immunofluorescence.- Methodology for Detecting and Tracking brain-Derived Neurotrophic Factor Complexes in Neurons using Single Quantum Dots.- Recording Activity-Dependent Release of BDNF from Hippocampal Neurons.- Ultrastructural Localization of BDNF and trkB Receptors.- Analysis of TrkB Receptor Activity using FRET Sensors.- BDNF-Induced Intracellular Signaling.- A Microfluidic Culture Platform for Neurotrophin Signaling Studies.- BDNF-Induced Local Protein Synthesis in Synaptoneurosomes Assessed with Click-iT L-azidohomoalanine.- Proteomic Tools to Study the Effect of BDNF on de novo Protein Synthesis.- BDNF Function in Long-Term Synaptic Plasticity in the Dentate Gyrus in Vivo: Methods for Local Drug Delivery and Biochemical Analysis of Translation.