Biomolecular Interactions Part B

• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in the Methods in Cell Biology series
• Updated release includes the latest information on biomolecular interactions instead of protein-protein interactions

1. Emerging Mechanisms of Targeted Protein Degradation by Molecular Glues 2. Design and use of programmable DNA Hydrogels 3. Oligomerization of membrane receptors: Approaches to measure in live cells 4. Interactions of alpha-synuclein with biomolecules 5. Gel-electrophoresis based method for biomolecular interaction 6. Recombinant centrosome expression in bacterial system 7. Reconstituting CCL5-CCR5 complex for structural and mechanistic analysis 8. Protein engineering and design in ion channel receptors 9. The crosstalk of G-proteinsand phospholipases in cellular signaling 10. Discovery of virus-host interactions using bioinformatics 11. Measuring Ligand-receptor interactions at opioid receptors 12. Label-free cell-based assays to unravel the biomolecular interactions involved in G-protein coupled receptor signaling 13. Measuring ligand-receptorinteractionsusing Atomic Force Microscopy 14. Reconstituting NTS1R signaling complex in lipid nanodisc 15. Proximity labeling methods to study biomolecular interactions 16. Super-resolution Microscopy approaches for monitoring protein trafficking and interaction 17. Interaction of cytosolic proteins with synthetic antibody fragments using NanoBiT assays 18. Reversible cross-linking to detect protein-protein interaction 19. Assays to monitor the activity of atypical chemokine receptors

Dr. Shukla subsequently carried out his post-doctoral work in the Department of Medicine at the Duke University in North Carolina, USA. During his post-doctoral research work, Dr. Shukla focused on understanding the biophysical and structural basis of ß-arrestin mediated regulation of GPCRs and non-canonical GPCR signaling. Dr. Shukla has served as an Assistant Professor in the Department of Medicine at the Duke University in Durham, North Carolina, USA.

Dr. Shukla is currently an Assistant Professor in Department of Biological Sciences and Bioengineering at the Indian Institute of Technology, Kanpur, India. Dr. Shukla is also an Intermediate Fellow of the Wellcome Trust-DBT India Alliance. The research program in Dr. Shukla's laboratory is focused on understanding the molecular mechanism of activation, signaling and regulation of G Protein-Coupled Receptors.