Bioimaging in Tissue Engineering and Regeneration Advanced Microscopy and Preclinical Imaging

This reference work gives a comprehensive overview about how to fully exploit the potential of bioimaging to characterize tissue regeneration holistically and to gain complementary, multi-scale information about relevant structure, function, dynamics and molecular composition. To keep pace with the highly dynamic nature of the field and fast-developing imaging technology, it is essential to allow its Live Reference version to be updated and dynamic. It guides the reader through real-case biomedical applications organized by chapter and gives a tissue-specific overview on how to best assess parameters of interest. Additionally, all imaging techniques, including emerging modalities, are explained in great detail and enables the reader to understand the physical principles behind each imaging modality. The book provides a unique overview of available biological and (pre)clinical imaging techniques in the field and enables the reader to understand their benefits and limitations for real-case applications. The reader will be equipped with a comprehensive tool to properly chose the most promising technique for her/his biomedical research question - and respond to novel trends due to the dynamic nature of the Live Reference version available online. The book is of high interest to all researchers, physicians, physicists and life scientists working in the field to deepen their theoretical and practical knowledge about bioimaging and about the wide range of tissue-specific biomedical applications. The book is a valuable reference in the field for bioimaging.

Fluorescence Microscopy of the Cell and Cell Interactions to Study Dynamic Cellular Processes.- Live Cell Imaging and in vivo Cell Tracking in Tissues.- Nonlinear Microscopy to Study Arthroscopy.- Synchronization to Visualization: Dissecting Myogenesis and Regeneration Using Correlative Light and Electron Microscopy (CLEM).- Raman Imaging for Biomedical Applications.- X-Ray-Based 3D Histo(patho)logy of Biological Tissues Using Contrast-Enhanced MicroCT.- Live Cell Imaging by High Resolution Quasi-Spectral Microscopy.- Confocal Microscopy Applications with Integrative Techniques in Cell Mechanics and Tissue Engineering.- Correlated Multimodal Imaging in Bone of Regeneration - A Showcase of Bisphosphonate-Treated Murine Jawbones.- Chemical Imaging in Bone and Cartilage Regeneration.- Quantitative Microstructural Imaging and Mechanics of Cartilage Using Experimental and Computational Approaches.- Osteoarthritis Imaging Using MRI.- Correlative Multimodal Imaging for Multiscale Analysis of Complex Biological Systems: Validation Methods and Performance Analysis.- HREM for Mesoscopic 3D Histology.- X-Ray Physics and Micromechanics-Guided Intravoxel Analysis of microCT-Imaged Hard Tissue Engineering Scaffolds and Bone.- Combined Imaging and Soft and Complex 3D Bioprinting Using Light Sheet Fluorescence Microscopy.

Prof Dr Andreas Walter studied physics and biophysics at the University of Heidelberg and in Sankt Petersburg on a Baden-Wuerttemberg Scholarship. He spent his research career developing and applying various imaging techniques for life sciences, including fluorescence, super-resolution, cryo-light, transmission electron and scanning electron microscopy, and correlative soft x-ray tomography.  After his tenure as a guest scientist at the European Molecular Biology Laboratory (EMBL) to study chromatin organization with fluorescence microscopy-based methods, he did his PhD at the Max Planck Institute of Biophysics in Frankfurt on method development in electron microscopy. He finished his studies with distinction (summa con laude). Prof Dr Walter received a DFG (German Research Association) Fellowship and a fellowship of the German Scholars Organization to continue his research in imaging at the University of California San Francisco and at the Lawrence Berkeley National Lab to correlate soft x-ray tomography and cryo-light microscopy. From 2016 till 2022, Prof Dr Walter worked as the Director of Austrian BioImaging where he coordinated more than 35 facilities and research groups for preclinical and biological imaging to develop and establish novel multimodal imaging pipelines. In 2022, Prof Dr Walter was appointed professor of Biophotonics and Bioimaging at Aalen University, Germany, where his group aims at developing novel correlative microscopy solutions to facilitate imaging across scales in life sciences (www.hs-aalen.de/zot-walter-lab). He also holds an MBA degree in Management of Research Infrastructures from the University of Milano-Bicocca. Up to 2024, Prof Dr Walter has published more than 35 peer-reviewed articles, and has been cited more than 1000 times on microscopy development to tackle a variety of biomedical research questions.

Paul Slezak leads a research group on soft tissue regeneration and local hemostasis at the Ludwig Boltzmann Institute for Traumatology in Vienna, Austria. He graduated in 2009 at the Medical University of Vienna and has a wide research interest,  spanning from the development of new therapies for soft tissue regeneration to designing next generation hemostats in the clinical field. He is member of the steering board of the Correlated Multimodal Imaging Node Austria (CMI) and Co-Lead at the newly founded Josef Ressel Centre for Materials Engineering in Soft Tissue Regeneration. Dr. Slezak is (co-)author of 54 peer-reviewed publications with an h-index of 24 and is the CEO of a Spin Off company which aims to bring experim