Autoradiography

Of interest to both students and biological scientists in a wide variety of research areas, this handbook provides a comprehensive overview of the major techniques of autoradiography. The reader will find detailed, practical instructions, as well as information on the rationale behind the methods, and a critical appraisal of autoradiographs. With this volume the author, a widely known expert in the field, provides an invaluable introduction to the theory and techniques of autoradiography and an important addition to the library of experienced researchers who are familiar with other methods, but would like to broaden the scope of their knowledge. The volume includes numerous illustrations and helpful lists of further readings.

This book describes levels of autoradiography, which is the localization within a solid specimen of a radiolabel, to help the beginner or biologist choose the correct approach for particular application. It attempts to lay emphasis on certain aspects of the appraisal of autoradiographs.

1. Introduction 2. Components of Autoradiographs 3. Macroautoradiography 4. Light Microscopic Autoradiography-Fixable Substances 5. Light Microscopic Autoradiography–Diffusible Substances 6. Autoradiography of Receptors 7. Electron Microscopic Autoradiography–Preparative Methods 8. Efficiency 9. Resolution 10. Quantification of EM Autoradiographs 11. Autoradiography in Relation to Other Techniques

John R.J. Baker