Asthma Methods and Protocols

This volume details a collection of laboratory protocols to study asthma in mice and humans. Chapters cover animal models of asthma, methods to measure asthma-related molecules, protocols to detect, isolate, culture and stimulate cells that contribute to asthma in mice and humans, methods to deplete these cells in vivo and study responses of intact airway tissues ex vivo. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Asthma: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

Modelling Asthma in Mice Using Common Aeroallergens.- Mouse Models of Respiratory Syncytial Virus Infection.- Modeling Asthma in Mice Using Rhinovirus Infection.- Mouse Model of Sendai Virus-induced Lung Disease.- Ozone-Induced Models of Airway Hyperreactivity and Epithelial Injury.- A Novel Non-Human Primate Model of Nonatopic Asthma.- Measurement of Airway Hyperresponsiveness in Mice.- Human Precision-Cut Lung Slices: Generation of and Measurement of Contractility and Relaxation of Small Airways.- Infection of Human Precision-Cut Lung Slices with the Influenza Virus.- Human Bronchial Epithelial Cell Culture Models for Cigarette Smoke and Vaping Studies.- Isolation, Ex Vivo Culture and Stimulation of Tracheal and Nasal Chemosensory Cells.- Detection, Isolation and Functional Studies of Mouse Pulmonary Group 2 Innate Lymphoid Cells.- Isolation and Characterization of Conventional and Non-Conventional Type-2 Innate Lymphoid Cells (ILC2s) from Human Peripheral Blood Mononuclear Cells (PBMCs).- Detection of Eosinophils in Tissue Sections by Immunohistochemistry.- Targeting Eosinophils in Mouse Models of Asthma.- Detection and Isolation of Airway Mast Cell Subsets in Mouse and Human.- Isolation of Conventional Murine Lung Dendritic Cell Subsets.- Isolation and Analysis of Macrophage Subsets from the Mouse and Human Lung.- Mass Cytometry Analysis of Whole Blood Response to an Allergen.- Methods to Detect Neutrophil Extracellular Traps in Asthma.- Analysis of Airway Vagal Neurons.- Protocols to Measure Oxidative Stress and DNA Damage in Asthma.