Arabidopsis Protocols

This fourth edition compiles the most recent methodologies developed to exploit the Arabidopsis genome. Chapters detail access to public resources, to genetic, cell biology, biochemical and physiological techniques, and sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Arabidopsis Protocols, Fourth Edition aims to ensure successful results in the further study of this vital field.

Handling Arabidopsis and other Brassicaceae: Growth, Preservation of Seeds, Transformation and Genetic Crosses.- Bioinformatic Tools in Arabidopsis Research.- Analyses of Natural Variation: Field Experiments and Nucleotide Diversity for Your Favorite Gene.- Non-sterile Grafting Methods for Arabidopsis.- CRISPR/Cas9-based Genome Editing Toolbox for Arabidopsis thaliana.- Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration.- Transient Expression of Fluorescent Fusion Proteins in Arabidopsis Protoplasts.- Photosynthetic and Heterotrophic Cell Suspension Cultures.- Genome-wide Association Studies in Arabidopsis thaliana: Statistical Analysis and Network-Based Augmentation of Signals.- Visualising and Measuring Single Locus Dynamics in Arabidopsis thaliana.- Identification and Quantification of Small RNAs.- Flow Cytometry and Sorting in Arabidopsis.- Live Imaging ofArabidopsis Leaf and Vegetative Meristem Development.- Using Genetically Encoded Fluorescent Biosensors for Quantitative in vivo Imaging.- Using Tripartite Split-sfGFP for the Study of Membrane Protein–Protein Interactions.- High-pressure Freezing and Freeze Substitution for Transmission Electron Microscopy Imaging and Immunogold-labeling.- Atomic Force Microscopy to Study Cell Wall Mechanics in Plants.- Identification and Characterization of Reproductive Mutations in Arabidopsis.- Metabolomic Analysis of Natural Variation in Arabidopsis.- Untargeted Metabolomics of Arabidopsis Stomatal Immunity.- Biotin-based Proximity Labeling Of Protein Complexes in Planta.- Phosphoproteomic Analysis of Plant Membranes.- RNA-binding Protein Immunoprecipitation and High Throughput Sequencing.