Animal Models of Allergic Disease Methods and Protocols

This volume provides protocols for mouse models of allergic diseases and guidelines for choosing a particular strains, allergen, adjuvant, and route of sensitization. Chapters detail types of allergic disease, methods that are frequently employed to analyze pathophysiology of allergic diseases, manipulation of intestinal microbiota, and desensitization of immure responses in animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Applications of Mouse Models to the Study of Food Allergy.- Induction of Peanut Allergy through Inhalation of Peanut in Mice.- Assessment of Immune Responses in an Animal Model of Wheat Food Allergy via Epicutaneous Sensitization.- A Mouse Model of Oral Sensitization to Hen’s Egg White.- Induction of Hypersensitivity with Purified Beta-Lactoglobulin as a Mouse Model of Cow’s Milk Allergy.- Diet-induced Mouse Model of Atopic Dermatitis.- Animal Models of Contact Dermatitis: 2,4-dinitrofluorobenzene-induced Contact Hypersensitivity.- Induction of Airway Hypersensitivity to Ovalbumin and Dust Mite Allergens as Mouse Models of Allergic Asthma.- Generation and Characterization of Inducible Lung and Skin-Specific IL-22 Transgenic Mice.- Experimental Mouse Models of Ragweed- and Papain-Induced Allergic Conjunctivitis.- Intravital Imaging of Vascular Permeability by Two-photon Microscopy.- Isotype-specific Detection of Serum Immunoglobulins against Allergens.- An Overview of Flow Cytometry: Its principles and applications in Allergic Disease Research.- The Application of Flow Cytometry for Simultaneous and Multi-Parametric Analysis of Heterogenous Cell Populations in Basic and Clinical Research.- Cellular and Biochemical Analysis of Bronchoalveolar Lavage Fluid from Murine Lungs.- Procedures to Evaluate Inflammatory and Pathological Changes during Allergic Airway Inflammation.- Assessment of Lung Eosinophils in situ using Immunohistological Staining.- Preservation and Processing of Intestinal Tissue for the Assessment of Histopathology.- Antibiotic Treatment in an Animal Model of Inflammatory Lung Disease.- Methods for Experimental Allergen Immunotherapy: Subcutaneous and Sublingual Desensitization in Mouse Models of Allergic Asthma.- T Cell Epitope Immunotherapy in Mouse Models of Food Allergy.