Animal Cell Culture and Technology

"The Basics" series provide the ideal introduction to new techniques in the laboratory. This series of books will allow you to use the techniques described, and to manipulate them to suit your own basic requirements.
This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for scientists intending to initiate some work in this field.
New topics are included to reflect the latest developments and trends in the field. They cover the latest theory of the biological clock (teleomeres) of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation and the humanization of antibodies for therapeutic use.

Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use.

1. Introduction: The Use of Animal Cell Culture 2. Characteristics of Cells in Culture 3. Basic Equipment and Laboratory Design: What You need to Get Started in Cell Culture 4. Growth and Maintenance of Cells in Culture 5. Cell Line and Culture Monitoring 6. Genetic Engineering of Animal Cells in Culture 7. The Glycosylation of Proteins in Cell Culture 8. Hybridomas - Sources of Antibodies 9. Scaling up Animal Cell Culture 10. Modes of Culture for High Cell Densities 11. Production from Cell Culture 12. Mammalian Cell Products: Established and Potential