Activity-Based Protein Profiling

This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions.

Activity-based protein pro?ling – enabling multimodal functional studies of microbial communities.- Activity-Based Protein Pro?ling Methods to Study Bacteria: The Power of Small-Molecule Electrophiles.- Opportunities and Challenges in Activity-Based Protein Pro?ling of Mycobacteria.- Activity-Based Protein Pro?ling at the Host–Pathogen Interface.- Chemical Proteomic Pro?ling of Protein Fatty-Acylation in Microbial Pathogens.- How to Target Viral and Bacterial Effector Proteins Interfering with Ubiquitin Signaling.- ABPP and Host–Virus Interactions.- Activity-Based Protein Pro?ling for the Study of Parasite Biology.- Deciphering T Cell Immunometabolism with Activity-Based Protein Pro?ling.- Small-Molecule Inhibitors of PARPs: From Tools for Investigating ADP-Ribosylation to Therapeutics.- Development of Activity-Based Proteomic Probes for Protein Citrullination.- Recent Advances in Activity-Based Protein Pro?ling of Proteases.- Opportunities for Lipid-Based Probes in the Field of Immunology.- Activity-Based Protein Pro?ling of Non-ribosomal Peptide Synthetases.- Target Identi?cation of Bioactive Covalently Acting Natural Products.- Applications of Reactive Cysteine Pro?ling.

Benjamin F. Cravatt, Ph.D.

Department of Chemistry

The Scripps Research Institute

La Jolla, CA, USA

cravatt@scripps.edu

Ku-Lung Hsu Ph.D.

University of Virginia School of Medicine

Department of Chemistry

Charlottesville, VA, USA

kh4ch@virginia.edu

Eranthie Weerapana Ph.D.

Boston College

Merkert Chemistry

Chestnut Hill, MA, USA

eranthie@bc.edu