Tissue Morphogenesis

TRAMA

Tissue Morphogenesis: Methods and Protocols highlights major techniques, both experimental and computational, for the study of tissue morphogenesis, divided into several sections, with specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis. Chapters focus on imagining analysis of tissue morphogenesis, culture models of tissue morphogenesis, manipulating cells and tissues in vivo, novel model systems to investigate issue morphogenesis and computational models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Tissue Morphogenesis: Methods and Protocols serves as a primary resource for both fundamental and practical understanding of the techniques used to uncover the basis of tissue morphogenesis.

SOMMARIO

PART I          IMAGING MORPHOGENESIS Chapter 1.       Probing Regional Mechanical Properties of Embryonic Tissue Using                                                Microindentation and Optical Coherence TomographyBenjamin A. Filas, Gang Xu, and Larry A. Taber Chapter 2.       Hemodynamic Flow Visualization of Early Embryonic Great Vessels using mPIVSelda Goktas, Chia-Yuan Chen, William J. Kowalski, and Kerem Pekkan Chapter 3.       Using Correlative Light and Electron Microscopy to Study Zebrafish Vascular MorphogenesisJacky G. Goetz, Fabien Monduc, Yannick Schwab, and Julien Vermot Chapter 4.       Micro/Nano-Computed Tomography Technology for Quantitative Dynamic, Multi-scale Imaging of Morphogenesis Chelsea L. Gregg, Andrew K. Recknagel, and Jonathan T. Butcher Chapter 5.       Imaging the Dorsal-Ventral Axis of Live and Fixed Drosophila Melanogaster EmbryosSophia N. Carrell and Gregory T. Reeves Chapter 6.       Light-Sheet-Based Imaging and Analysis of Early Embryogenesis in the Fruit FlyKhaled Khairy, William Lemon, Fernando Amat, and Philipp J. Keller Chapter 7.       Quantitative Image Analysis of Cell Behavior and Molecular Dynamics During Tissue Morphogenesis Chun Yin Bosco Leung and Rodrigo Fernandez-Gonzalez Chapter 8.       A Multiplex Fluorescent in situ Hybridization Protocol for Clonal Analysis of Drosophila Oogenesis Lily S. Cheung and Stanislav Shvartsman Chapter 9.       Active Cell and ECM Movements During DevelopmentAnastasiia Aleksandrova, Brenda J. Rongish, Charles D. Little, and Andras Czirok PART II         CULTURE MODELS OF MORPHOGENESIS Chapter 10.     3D Culture Assays of Murine Mammary Branching Morphogenesis and Epithelial InvasionKim-Vy Nguyen-Ngoc, Eliah R. Shamir, Robert J. Huebner, Jennifer N. Beck, Kevin J. Cheung, and Andrew J. Ewald Chapter 11.     Culture of Mouse Embryonic Foregut Explants Felicia Chen and Wellington V. Cardoso Chapter 12.     Investigating Human Vascular Tube Morphogenesis and maturation using  endothelial cell-pericyte co-cultures and a doxycycline-inducible genetic system in 3D extracellular matrices Stephanie L.K. Bowers, Chun-Xia Meng, Matthew T. Davis, and George E. Davis Chapter 13.     Three-Dimensional Traction Force Microscopy of Engineered Epithelial Tissues Alexandra S. Piotrowski, Victor D. Varner, Nikolce Gjorevski, and Celeste M. Nelson PART III       MANIPULATING CELLS AND TISSUES IN VIVOChapter 14.     Probing Cell Mechanics with Sub-Cellular Laser Dissection of Actomyosin  Networks in the Early Developing Drosophila Embryo M. Rauzi and P.-F. Lenne Chapter 15.     UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish    Embryo in vivo and ex vivoMichael Smutny, Martin Behrndt, Pedro Campinho, Verena Ruprecht, and Carl-Philipp Heisenberg Chapter 16.     Measurement of Intercellular Cohesion by Tissue Surface Tension Ramsey A. Foty Chapter 17.     Quail-Chick Chimeras and Eye Development Sinu Jasrapuria Agrawal and Peter Y. Lwigale PART IV       EMERGING MODELS OF TISSUE MORPHOGENESIS Chapter 18.     Studying Epithelial Morphogenesis in Dictyostelium Daniel J. Dickinson, W. James Nelson, and William I. Weis Chapter 19.     Primary Cell Cultures of Regenerating Holothurian Tissues Samir A. Bello, Ricardo J. Abreu-Irizarry, and Jose E. Garcia-Arraras PART V         COMPUTATIONAL MODELS OF TISSUE MORPHOGENESIS Chapter 20.     Large-Scale Parameter Studies of Cell-Based Models of Tissue Morphogenesis   Using CompuCell3D or VirtualLeafMargriet M. Palm and Roeland M. H. Merks Chapter 21.     Simulating Tissue Morphogenesis and SignalingDagmar Iber, Simon Tanaka, Patrick Fried, Philipp Germann, and Denis  Menshykau Chapter 22.     Elasticity-Based Targeted Growth Models of Morphogenesis Patrick W. Alford

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781493911639
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 9089 gr
• Formato: Copertina rigida
• Illustration Notes: XII, 351 p. 106 illus., 76 illus. in color. With online files/update.
• Pagine Arabe: 351
• Pagine Romane: xii