Biothermodynamics

NOTE EDITORE

This book covers the fundamentals of the rapidly growing field of biothermodynamics, showing how thermodynamics can best be applied to applications and processes in biochemical engineering. It describes the rigorous application of thermodynamics in biochemical engineering to rationalize bioprocess development and obviate a substantial fraction of this need for tedious experimental work. As such, this book will appeal to a diverse group of readers, ranging from students and professors in biochemical engineering, to scientists and engineers, for whom it will be a valuable reference.

SOMMARIO

FundamentalsTHE ROLE OF THERMODYNAM ICS IN BIOCHEMICAL ENGINEERINGBasic remarks on thermodynamics in biochemical engineeringFundamental concepts in equilibrium thermodynamicsCharged species, gels and other soft systemsStability and activity of biomacromoleculesThermodynamics of live cellsThermodynamic analysis of metabolismConclusionsReferencesPHASE EQUILIBRIUM IN NON-ELECTROLYTE SYSTEMSIntroductionEssential formal relations1 Criteria for equilibriumLiquid-liquid equilibriaSolid-liquid equilibriaReferencesVirial Expansion for Chemical Potentials in a Dilute Solution for Calculation of Liquid-Liquid EquilibriaIntroductionExample of protein separationReferencesThe thermodynamics of electrically charged molecules in solutionWhy do electrically charged molecules call for a particular thermodynamic treatment?The thermodynamics of electrolytesElectrostaticsEmpirical and advanced ion activity coefficient modelsReferencesWATERIntroductionPhenomenological aspects of waterM olecular properties of waterWater as a solventFurther readingCharged Species, Gels, and other Soft SystemsPOLYMERS, POLYELECTROL YTES AND GELSFlory’s Theory of polymer solutionsElectric Charge on a weak polyelectrolyteHydrogels: Elementary Equations for Idealized Networks and Their Swelling BehaviorAppendix: Entropy of mixing for polymer solutionsReferencesSELF-ASSEMBLY OF AMPHIPHILIC MOLECULESIntroductionSelf-assembly as phase separationDifferent types of self-assembled structuresAggregation as a "start-stop" process: size and shape of self-assembled structuresMass action model for micellizationFactors that influence the critical micelle concentrationBilayer structuresReverse micellesMicroemulsionsSelf-assembled structures in applicationsReferencesMOLECULAR THERMODYNAMICS OF PARTITIONING IN AQUEOUS TWO-PHASE SYSTEMSIntroductionFlory–Huggins theory applied to aqueous two-phase partition systemsDependence of partitioning on system variablesSimple interpretation of the effects of added electrolyteCalculation of phase diagrams and partitioningConclusionsReferencesGENERALIZATION OF THERMODYNAMIC PROPERTIES FOR SELECTION OF BIOSEPARATION PROCESSESPhase behavior in Bioseparation ProcessesGeneralized correlationGeneralized polarity scalesConclusionsAPPENDIXReferencesProtein Precipitation with Salts and/or PolymersIntroductionEquation of stateThe potential of mean forcePrecipitation calculationsGeneralization to a multicomponent solution.CrystallizationReferencesMULTICOMPONENT ION EXCHANGE EQUILIBRIA OF WEAK ELECTROLYTE BIOMOLECULESIntroductionMulti-component ion exchange of weak electrolytesExperimental case studiesConclusionsReferencesStability and Activity of BiomacromoleculesPROTEINSIntroductionThe amino acids in proteinsThe three-dimensional structure of protein molecules in aqueous solutionNon-covalent interactions that determine the structure of a protein molecule in waterStability of protein structure in aqueous solutionThermodynamic analysis of protein structure stabilityReversibility of protein denaturation aggregation of unfolded protein moleculesReferencesTHERMODYNAMICS IN MULTIPHASE BIOCATALYSISWhy multiphase biocatalysis?Thermodynamics of enzymatic reactions in aqueous systemsNon-aqueous media for biocatalysis.Using enyzmes in organic solventsPhase equilibria in multiphase enyzmatic reactionsWhole cells in organic solventsList of symbolsReferencesThermodynamics of the Physical Stability of Protein SolutionsIntroductionFactors influencing protein stabilityMechanism of protein aggregationSummary and conclusionsReferencesMeasuring, Interpreting and Modeling the Stabilities and Melting Temperatures of B-Form DNA s that Exhibit a Two-State Helix-to-Coil TransitionIntroductionMethods for measuring duplex DNA melting thermodynamicsModeling dsDNA stability and the melting transitionComparing and further improving the performance of NNT modelsFinal thoughtsReferencesThermodynamics in Living SystemsLIVE CELLS AS OPEN NON-EQUILIBRIUM SYSTEMSIntroductionBalances for open systemsEntropy production, forces and fluxesFlux-force relationships and coupled processesThe linear energy converter as a model for living systemsConclusionsReferencesMiniaturization of Calorimetry: Strengths and Weaknesses for Bioprocess Monitoring and ControlWhy miniaturization of calorimeters?Historical rootsMeasurement principleCalorimetry versus off-gas analysisApplications of chip-calorimetryOutlookReferencesA thermodynamic approach to predict Black Box model parameters for microbial growthIntroductionCatabolic energy productionThermodynamic prediction of the parameters in the Herbert-Pirt substrate distribution relationPrediction of the qp(?) relationshipPrediction of the process reactionPrediction of the hyperbolic substrate uptake kinetic parametersInfluence of temperature and pH on Black Box model parametersHeat production in biological systemsConclusionReferencesFurther readingBIOTHERMODYNAMICS OF LIVE CELLS:Energy dissipation and heat generation in cellular culturesWhy study heat generation and energy dissipation in biotechnology?The first law: measuring, interpreting and exploiting heat generation in live culturesThe second law: energy dissipation, driving force and growthPredicting energy and heat dissipation by calculationResults: heat generation and Gibbs energy dissipation as a function of biomass yieldApplication: prediction of yield coefficientsDiscussion and conclusionsAppendix: Example calculation for prediction of growth stoichiometryReferencesTHERMODYNAMIC ANALYSIS OF PHOTOSYNTHESISIntroductionReferencesThermodynamics of MetabolismA THERMODYNAMIC ANALYSIS OF DICARBOXYLIC ACID PRODUCTION IN MICROORGANISMSIntroductionOutline of the approachThermodynamics of dicarboxylic acid transportGenetic engineering of target systems based upon thermodynamic analysis resultsConclusion.AppendicesReferencesTHERMODYNAMIC ANALYSIS OF METABOLIC PATHWAYSIntroductionThermodynamic feasibility analysis of individual metabolic pathwaysEstimation of observable standard Gibbs energies of reactionMaterials and methods [22]Results and discussionConclusionsReferencesIndex.

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781466582163
• Dimensioni: 9 x 6 in Ø 2.80 lb
• Formato: Copertina rigida
• Pagine Arabe: 380