The Nucleus

TRAMA

This detailed new edition delves into recently developed protocols for those working on cell nucleus research, including some of the less well-explored areas of study. Like the previous editions, this book features contributions from top experts in their respective fields. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, The Nucleus, Third Edition will help students and postdocs become aware of vital available methodologies when planning their new projects.

SOMMARIO

1. Live-Cell Imaging and Analysis of Nuclear Body Mobility             Dmitry V. Sorokin, Eugene A. Arifulin, Yegor S. Vassetzky, and Eugene V. Sheval   2. Laser Targeted Oligo Ligation (LTOL) to Identify DNA Sequences in the Vicinity of a Single Subnuclear Structure in a Single Cell             David Anchel and Reagan W. Ching   3. Visualizing Chromatin Modifications in Isolated Nuclei             Yuan Li, Zachary Klase, and Luca Sardo   4. Dual-Color Metal-Induced Energy Transfer (MIET) Imaging for Three-Dimensional Reconstruction of Nuclear Envelope Architecture             Alexey I. Chizhik, Anna M. Chizhik, Daja Ruhlandt, Janine Pfaff, Narain Karedla, Ingo Gregor, Ralph H. Kehlenbach, and Jörg Enderlein   5. Studying Proton Gradients Across the Nuclear Envelope             Raul Martínez-Zaguilán and Souad R. Sennoune   6. COMBinatorial Oligonucleotide FISH (COMBO-FISH) with Uniquely Binding Repetitive DNA Probes             Michael Hausmann, Jin-Ho Lee, Aaron Sievers, Matthias Krufczik, and Georg Hildenbrand   7. Genome-Wide Mapping of UV-Induced DNA Damage with CPD-Seq             Peng Mao and John J. Wyrick   8. AP-Seq: A Method to Measure Apurinic Sites and Small Base Adducts Genome-Wide             Anna R. Poetsch   9. Locus-Specific Chromatin Proteome Revealed by Mass Spectrometry-Based CasID             Enes Ugur, Michael D. Bartoschek, and Heinrich Leonhardt   10. Methyl Adenine Identification (MadID): High-Resolution Detection of Protein-DNA Interactions             David Umlauf, Michal Sobecki, and Laure Crabbe   11. Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres             Šárka Schorová, Jirí Fajkus, and Petra Procházková Schrumpfová   12. Macromolecular Crowding Measurements with Genetically Encoded Probes Based on Förster Resonance Energy Transfer in Living Cells             Sara N. Mouton, Liesbeth M. Veenhoff, and Arnold J. Boersma   13. Analysis of a Nuclear Intrinsically Disordered Proteome             Bozena Skupien-Rabian, Urszula Jankowska, and Sylwia Kedracka-Krok   14. Timing of Cytosine Methylation on Newly-Synthesized RNA by Electron Microscopy             Lorena Zannino, Stella Siciliani, and Marco Biggiogera   15. The Nucleus of Intestinal Cells of the Bacterivore Nematode Caenorhabditis elegans as a Sensitive Sensor of Environmental Pollutants             Annette Piechulek, Lutz Berwanger, Peter Hemmerich, and Anna von Mikecz

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781071607657
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 448 gr
• Formato: Brossura
• Illustration Notes: XI, 220 p. 42 illus., 30 illus. in color.
• Pagine Arabe: 220
• Pagine Romane: xi