T-Helper Cells

TRAMA

The aim of this volume is to provide a comprehensive description of methods and protocols useful for the further study of T-helper cells. Chapters guide readers through T-helper cell recovery, molecular study, signal transduction pathways, T-cell manipulation and, last but not least, “omic” approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T- Helper Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

SOMMARIO

1. Human and Murine T-Helper Cell Recovery from Organs and Tissues Francesco Siracusa, Annette Lahmann, Anna Vanni, Filippo Cortesi, and Alessio Mazzoni   2. Identification and Purification of Human Memory T-Helper Cells from Peripheral Blood Alessia Capone and Elisabetta Volpe   3. Transcription Factor Evaluation by Flow Cytometry Daniela  Fenoglio and  Tiziana Altosole   4. Mass Cytometry Analysis of T-Helper Cells Priyanka B. Subrahmanyam and Holden T. Maecker   5. Murine T-Helper Cell Differentiation and Plasticity Francesco Siracusa, Franziska Muscate, and Laura Garcia Perez   6. Identification of Follicular T-Cell Subsets in Murine and Human Tissues Annette Lahmann, Laura Bauer, and Andreas Hutloff   7. Analysis of T-Cells In Inflamed Non-Lymphoid Tissues Laura Bauer and Andreas Hutloff   8. TCR Vß Evaluation by Flow Cytometry Benedetta Peruzzi, Sara Bencini, and Roberto Caporale   9. Evaluation of Cytokine Production at the Single Cell Level by Flow Cytometry Upon Polyclonal Stimulation Jakob Zimmermann   10. Evaluation of Secreted Cytokines by Multiplex Bead Based Assay (X MAP Technology, Luminex) Letizia Lombardelli, Federica Logiodice, Ornela Kullolli, and Marie-Pierre Piccinni   11. Quantifying Antigen-specific T-Cells by Assessing their Antigen-Induced Proliferation Aayushi Shah and Hyun-Dong Chang   12. Flow Cytometric Characterization of Human Antigen-Reactive T-Helper Cells Carina Saggau, Alexander Scheffold, and Petra Bacher   13. Identification of Human Antigen-Specific CD4+ cells with Peptide-MHC Multimer Technologies I-Ting Chow and William W. Kwok   14. Human T-cell Cloning by Limiting Dilution Laura Maggi, Manuela Capone, and Alessio Mazzoni   15. Human Germinal Center Reaction: Assessing T-Follicular Helper-B cell Interactions In Vitro Emeline Thevenon, Vanessa Cornacchione, Elisabetta Traggiai, and Francesca Rucci   16. Study of Signal Transduction Pathways by Phospho-Protein Evaluation Manuela Capone, Alessio Mazzoni, and Laura Maggi   17. The Study of Protein-DNA Interactions in CD4+ T Cells using ChIPmentation Arnulf Hertweck and Richard G. Jenner   18. mRNA Transfection of T lymphocytes by Electroporation Lea-Isabell Schwarze and Boris Fehse   19. Transduction of Human T-Cell Subsets with Lentivirus Vivian C. W. Fung, Isaac Rosado-Sánchez, Megan K Levings   20. Plasmid- or Ribonucleoprotein-mediated CRISPR/Cas Gene Editing in Primary Murine T-Cells Marianne Dölz, Romina Marone, and Lukas T. Jeker   21. Generation of Sequencing Libraries for Building Immune Cell Methylomes Stefan Floess and Jochen Huehn   22. Gene Expression Analysis of T-Cells by Single Cell RNA-seq Domenico Lo Tartaro, Sara De Biasi, Mattia Forcato, Lara Gibellini, and Andrea Cossarizza   23. Quantifying Proteome and Protein Modifications in Activated T-Cells by Multiplexed Isobaric Labeling Mass Spectrometry Haiyan Tan, Daniel Bastardo Blanco, Boer Xie, Yuxin Li, Zhiping Wu, Hongbo Chi, and Junmin Peng   24. Metabolomics, Lipidomics and Immunometabolism Fortunata Carbone, Sara Bruzzaniti, Clorinda Fusco, Alessandra Colamatteo, Teresa Micillo, Paola de Candia, Fabrizia Bonacina, Giuseppe Danilo Norata and Giuseppe Matarese   25. Experimental Mouse Models of Asthma and Analysis of CD4+ T Cells William J. Branchett, Simone A. Walker, and Clare M. Lloyd   26. Murine T-Cell Transfer Colitis as a Model for Inflammatory Bowel Disease Patrick Maschmeyer, Jakob Zimmermann, and Anja Andrea Kühl   27. Experimental Autoimmune Encephalomyelitis Clara Ballerini    

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781071613139
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 758 gr
• Formato: Brossura
• Illustration Notes: XII, 388 p. 103 illus., 51 illus. in color.
• Pagine Arabe: 388
• Pagine Romane: xii