Spliceosomal Pre-mRNA Splicing

TRAMA

Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of alternative pre-mRNA splicing in seven introductory chapters and then introduces protocols and their theoretical background relevant for a variety of experimental research. These protocol chapters cover basic methods to detect splicing events, analyses of alternative pre-mRNA splicing in vitro and in vivo manipulation of splicing events and high-throughput and bioinformatic analyses of alternative splicing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, Spliceosomal Pre-mRNA Splicing: Methods and Protocols will aid newcomers and seasoned molecular biologists in understanding the fascinating world of alternative splicing with the ultimate goal of paving the way for many new discoveries to come.

SOMMARIO

Part I: Introductory Chapters 1. The Pre-mRNA Splicing Reaction            Somsakul Wongpalee and Shalini Sharma2. Diversity and Evolution of Spliceosomal Systems            Scott William Roy and Manuel Irimia3. Mechanisms of Spliceosomal Assembly            Ni-ting Chiou and Kristen W. Lynch4. Alternative Pre-mRNA Splicing            Stacey D. Wagner and J. Andrew Berglund5. Regulation of Alternative Pre-mRNA Splicing            Miguel B. Coelho and Christopher W.J. Smith6. Introduction to Co-Transcriptional RNA Splicing            Evan C. Merkhofer, Peter Hu, and Tracy L. Johnson7. Chromatin and Splicing            Nazmul Haque and Shalini OberdoerfferPart II: Methods Chapters8. Preparation of Splicing Competent Nuclear Extracts            Chiu-Ho T. Webb and Klemens J. Hertel9. Preparation of Yeast Whole Cell Splicing Extract            Elizabeth A. Dunn and Stephen D. Rader10. Efficient Splinted Ligation of Synthetic RNA Using RNA Ligase            Martha R. Stark and Stephen D. Rader11. In Vitro Assay of Pre-mRNA Splicing in Mammalian Nuclear Extract            Maliheh Movassat, William F. Mueller, and Klemens J. Hertel12. Kinetic Analysis of In Vitro Pre-mRNA Splicing in HeLa Nuclear Extract            William F. Mueller and Klemens J. Hertel13. In Vitro Systems for Coupling RNAP II Transcription to Splicing and Polyadenylation            Eric G. Folco and Robin Reed14. Isolation and Accumulation of Spliceosomal Assembly Intermediates            Janine O. Ilagan and Melissa S. Jurica15. Complementation of U4 snRNA in S. cerevisiae Splicing Extracts for Biochemical Studies of snRNP Assembly and Function            Martha R. Stark and Stephen D. Rader16. Expression and Purification of Splicing Proteins from Mammalian Cells            Eric Allemand and Michelle L. Hastings17. Single Molecule Approaches for Studying Spliceosome Assembly and Catalysis            Eric G. Anderson and Aaron A. Hoskins18. Cell-Based Splicing of Minigenes            Sarah A. Smith and Kristen W. Lynch19. Quantifying the Ratio of Spliceosome Components Assembled on Pre-mRNA            Noa Neufeld, Yehuda Brody, and Yaron Shav-Tal20. Antisense Methods to Modulate Pre-mRNA Splicing            Joonbae Seo, Eric W. Ottesen, and Ravindra N. Singh21. Using Yeast Genetics to Study Splicing Mechanisms            Munshi Azad Hossain and Tracy L. Johnson22. Medium Throughput Analysis of Alternative Splicing by Fluorescently Labeled RT-PCR            Ryan Percifield, Daniel Murphy, and Peter Stoilov23. Chromatin Immunoprecipitation Approaches to Determine Co-Transcriptional Nature of Splicing            Nicole I. Bieberstein, Korinna Straube, and Karla M. Neugebauer24. Computational Approaches to Mine Publicly Available Databases            Rodger B. Voelker, William A. Cresko, and J. Andrew Berglund25. Approaches to Link RNA Secondary Structures with Splicing Regulation            Mireya Plass and Eduardo Eyras26. Methods to Study Splicing from High-Throughput RNA Sequencing Data            Gael P. Alamancos, Eneritz Agirre, and Eduardo Eyras27. Global Protein-RNA Interaction Mapping at Single Nucleotide Resolution by iCLIP-Seq            Chengguo Yao, Lingjie Weng, and Yongsheng Shi28. Predicting Alternative Splicing            Yoseph Barash and Jorge Vaquero Garcia

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781627039796
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 9659 gr
• Formato: Copertina rigida
• Illustration Notes: XI, 427 p. 66 illus., 34 illus. in color.
• Pagine Arabe: 427
• Pagine Romane: xi