Lipid Analysis

TRAMA

There is increasing recognition of the diversity and biological importance of lipids. Lipid modifications of other biological molecules are now also the subject of intense research activity. This book begins by describing the techniques currently used in the analysis of lipids and lipid modifications such as: * Extraction * Chromatographic and spectroscopic methods * Radioisotopic and immunochemical methods The application of these techniques to detection and determination of structure are then described for the major group of lipidsincluding: * Fatty acids and prostaglandins * Phospholipids and related compounds * Glycolipids and glycoproteins Lipid Analysis essential reading doe newcomers to this field and a useful update for more experienced workers.

SOMMARIO

Abbreviations -- Preface -- 1. Introduction -- 2. Basic Techniques -- Extraction methods -- Lipid binding in tissues -- Solvents for lipid extraction -- Some precautions -- Storage of lipid extracts -- Saponification -- Chromatographic procedures -- General considerations -- Partition chromatography -- Adsorption chromatography -- Ion exchange chromatography -- Complexing chromatography -- Spectrometric methods -- UV-visible spectrometry -- IR spectroscopy -- NMR spectroscopy -- Mass spectrometry -- Radioisotopic methods -- Choice of radioisotope -- Autoradiography -- Scintillation counting -- Gas ionization detection -- Gamma counting -- Radioisotopes in lipid studies -- RIA and related techniques -- Immunochemical methods -- Antibodies to lipids -- Hemagglutination in detection and assay -- Immunostaining and TLC -- Immunoradiometric assays -- Enzyme-linked immunosorbent assays -- Analytical methods involving enzymes -- Phospholipases -- Stereospecific analysis of triacylglycerol -- Enzymes in quantifying lipids -- Presentation of lipids in aqueous systems -- Care and safety during the analysis of lipids in biological materials -- General considerations -- Chemical hazards -- Fire hazards -- Radioisotope hazards -- Biological hazards -- Other hazards -- Personal preparedness and protection -- References -- 3. Hydrocarbons -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- References -- 4. Alcohols, Phenols, Aldehydes, Ketones and Quinones -- Alcohols and phenols -- General and biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Aldehydes and ketones -- General and biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Quinones -- General and biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- References -- 5. Fatty Acids and Prostaglandins -- Fatty acids -- Types of fatty acids found naturally -- Separation methods for fatty acids -- GLC of fatty acids -- HPLC of fatty acids -- Prostaglandins and leukotrienes (eicosanoids) -- Analysis of eicosanoids -- References -- 6. Esters -- Acylglycerols -- Separation of acylglycerols -- Analysis of acylglycerols -- Fatty acids of acylglycerols -- Wax esters -- Alkylglycerols -- Analytical methods for alkylglycerols -- References -- 7. Phospholipids, Sulfolipids and Related Compounds -- Phospholipids with amino-groups -- Hydrolytic procedures in analysis -- Estimation of phosphate -- Separation of phospholipids -- Phosphoinositides -- Phosphoinositide and inositol phosphate metabolism -- Separation of the phosphoinositides -- Receptor-linked phosphoinositide changes -- Separation and estimation of inositol phosphates -- Estimation of diacylglycerol -- Phosphatidylinositol glycans -- Detection of PI glycans -- Phosphonolipids -- Separation and analysis of phosphonolipids -- Alkylether phospholipids -- Analysis of alkylether phospholipids -- Sulfolipids -- Lipid sulfates -- Sulfonolipids -- Analytical methods -- References -- 8. Glycolipids -- Glycosphingolipids -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Polyisoprenyl phosphosugars -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Glycosylglycerides and related compounds -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Phosphoglycolipids, lipoteichoic acids and related compounds -- Biological significance -- Structures -- Detection, isolation, preparation and quantitation -- References -- 9. Lipoproteins -- Plasma lipoproteins -- Structures and biological significance -- Detection -- Isolation and purification -- Quantitation -- Fatty acyl proteins and prenyl proteins -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- References -- Appendices -- Appendix A: Glossary -- Appendix B: Suppliers -- Appendix C: Further reading -- Index.

AUTORE

Department of Biochemistry, Medical School, Queen's Medical Centre, Nottingham NG7 2UH, UK. Department of Biochemistry, Medical School, Queen's Medical Centre, Nottingham NG7 2UH, UK.

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781872748986
• Dimensioni: 9.25 x 6.25 in Ø 0.75 lb
• Formato: Brossura
• Pagine Arabe: 192