Human Naïve Pluripotent Stem Cells

TRAMA

This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Human Naïve Pluripotent Stem Cells: Methods and Protocols is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field.

SOMMARIO

Preface…Table of Contents…Contributing Authors...1. Deriving Human Naïve Embryonic Stem Cell Lines from Donated Supernumerary Embryos using Physical Distancing and Signal InhibitionStanley E. Strawbridge, James Clarke, Ge Guo, and Jennifer Nichols2. Induction of Human Naïve Pluripotency Using 5i/L/A MediumLaura A. Fischer, Shafqat A. Khan, and Thorold W. Theunissen3. Induction of Human Naïve Pluripotency Using Chemical ResettingPeter J. Rugg-Gunn4. Induction of Human Naïve Pluripotent Stem Cells from Somatic CellsConstance Onfray, Jia Ping Tan, Stéphanie Kilens, Xiaodong Liu, Jose Polo, and Laurent David5. Using Microfluidics to Generate Human Naïve and Primed Pluripotent Stem CellsIrene Zorzan, Onelia Gagliano, Nicola Elvassore, and Graziano Martello6. Maintenance of Human Naïve Pluripotent Stem CellsMaria Rostovskaya7. Generating Trophoblast Stem Cells from Human Naïve Pluripotent Stem CellsChen Dong and Thorold W. Theunissen8. Differentiation and Expansion of Human Extra-Embryonic Endoderm Cell Lines from Naïve Pluripotent Stem CellsMadeleine Linneberg-Agerholm and Joshua Mark Brickman9. Capacitation of Human Naïve Pluripotent Stem CellsMaria Rostovskaya10. Generation of Pericytic-Vascular Progenitors from Tankyrase/PARP-Inhibitor-Regulated Naïve (TIRN) Human Pluripotent Stem CellsLudovic Zimmerlin, Tea Soon Park, Imran Bhutto, Gerard Lutty, and Elias T. Zambidis11. Profiling DNA Methylation in Human Naïve Pluripotent Stem CellsFerdinand von Meyenn12. Chromatin Profiling of Human Naïve Pluripotent Stem CellsAdam Bendall and Claudia I. Semprich13. Mapping Chromatin Accessibility in Human Naïve Pluripotent Stem Cells using ATAC-seqJessica K. Cinkornpumin, Ishtiaque Hossain, and William A. Pastor14. Transcriptomic Analysis of Human Naïve and Primed Pluripotent Stem CellsArindam Ghosh and Anup Som15. Study of X Chromosome Activity Status in Human Naïve Pluripotent Stem Cells using RNA-FISH Kasturi Mahadik and Claire Rougeulle16. Flow Cytometry Analysis of Cell-Surface Markers to Identify Human Naïve Pluripotent Stem CellsPeter J. Rugg-Gunn17. Characterizing the Genetic Stability of Human Naïve and Primed Pluripotent Stem CellsDuncan Baker and Ivana BarbaricSubject Index List…

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781071619070
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 765 gr
• Formato: Copertina rigida
• Illustration Notes: X, 288 p. 66 illus.
• Pagine Arabe: 288
• Pagine Romane: x