Bifidobacteria

TRAMA

This volume provides current protocols that can be used in various experimental settings involving bifidobacteria. Chapters guide readers through experimental protocols on procedures to isolate and cultivate bifidobacteria, taxonomic identification of bifidobacterial isolates, sequencing and annotate genomes, physiologically characterize bifidobacteria, and methods on the genetic manipulation of bifidobacterial strains.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge,  Bifidobacteria: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

SOMMARIO

1. Methods for the Isolation and Recovery of Bifidobacteria Abelardo Margolles and Lorena Ruiz   2. Bifidobacterium Transformation Emily C. Hoedt, Roger S. Bongers, Francesca Bottacini, Jan Knol, John MacSharry, and Douwe van Sinderen   3. Isolation of Chromosomal and Plasmid DNA from Bifidobacteria María Esteban-Torres, Lorena Ruiz, Rocío Sánchez-Gallardo, and Douwe van Sinderen   4. Assembly, Annotation, and Comparative Analysis of Bifidobacterial Genomes Gabriele Andrea Lugli   5. Site-directed Mutagenesis of Bifidobacterium Strains Kieran James and Douwe van Sinderen   6. Protocol to Select Bifidobacteria from Fecal and Environmental Samples Giulia Alessandri, Maria Cristina Ossiprandi, Marco Ventura, and Douwe van Sinderen   7. Phageome Analysis of Bifidobacteria-Rich Samples Brian McDonnell, Eoghan Casey, Christian Milani, Gabriele Andrea Lugli, Alice Viappiani, Jennifer Mahony, Marco Ventura, and Douwe van Sinderen   8. Measuring Conjugated Linoleic Acid (CLA) Production by Bifidobacteria Grace Ahern, Douwe van Sinderen, Bo Yang,  R. Paul Ross, and Catherine Stanton   9. Detection, Isolation, and Purification of Bifidobacterial Exopolysaccharides Patricia Ruas Madiedo   10. Determination of Bifidobacterial Carbohydrate Utilisation Abilities and Associated Metabolic End-Products Ana Solopova and Douwe van Sinderen   11.Model for Murine Gut Colonisation by Bifidobacteria Valerio Rossini and Ken Nally   12. Identification of Bifidobacteria by the Phosphoketolase Assay Monica Modesto, Alice Checcucci, and Paola Mattarelli   13. Determination of Bile Salt Hydrolase Activity in Bifidobacteria Lorena Ruiz, Borja Sánchez, and Abelardo Margolles   14. A Resource for Cloning and Expression Vectors Designed for Bifidobacteria: Overview of Available Tools and Biotechnological Applications Lorena Ruiz, María Esteban-Torres, and Douwe van Sinderen   15. Metagenomic Analyses of Bifidobacterial Communities Christian Milani   16. Resistance of Bifidobacteria toward Antibiotics Miguel Gueimonde and Silvia Arboleya   17. In vitro Assessment of Prebiotic Activity Alberto Amaretti, Stefano Raimondi, Nicola Volpi, and Maddalena Rossi   18. Bifidobacterium Genome Assembly and Methylome Analysis using Pacbio SMRT Sequencing Francesca Bottacini and Douwe van Sinderen        

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781071612767
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 485 gr
• Formato: Brossura
• Illustration Notes: X, 239 p. 26 illus., 18 illus. in color.
• Pagine Arabe: 239
• Pagine Romane: x