Basic Methods in Antibody Production and Characterization

NOTE EDITORE

First published in 2001: Written for researchers and professionals in the fields of biomedical research, immunology, biochemistry, molecular biology, pathology, and biotechnology, Basic Methods in Antibody Production and Characterization uses a cookbook approach to presenting the methods for the production, characterization, and use of antibodies. Antibodies described include polyclonal and monoclonal and those made by the phage display. Basic Methods in Antibody Production and Characterization focuses on specific aspects of antibodies and takes readers on a step-by-step process from antigen preparation to immunizations, adjuvants, screening, purification, storage, and applications.Introductory material accompanies each chapter, giving readers a better understanding of the methods and applications. Ample references arm researchers with many applications pertaining to the particular technology.

SOMMARIO

1. Starting Out Delia R. Bethell and Gary C. Howard 2. Animal Welfare And Regulatory Issues Sigmund T. Rich 3. Antigens Donald H. Lewis 4. Adjuvants Jory R. Baldridge and Michael J. Lacy 5. Polyclonal Antibodies Eric S. Bean 6. Monoclonal Antibody Production Sandy J. Stewart 7. Screening Techniques Gamal E. Osman and Warren E. Ladiges 8. Antibody Production By Ascites Growth Kristi R. Harkins 9. Growth Of Cell Lines In Bioreactors Beverly J. Norris, Michael J. Gramer, and Mark Hirschel 10. Phage Display Peter Amersdorfer and James D. Marks 11. Antibody Purification Methods Kristi R. Harkins 12. Molecular Characterization Of Immunoglobulin Genes Warren Ladiges and Gamal E. Osman 13. Antibody Storage Kathryn Elwell and Delia R. Bethell 14. Chemically Modifying Antibodies Kathryn Elwell and Gary C. Howard 15. Western Blotting Lee Bendickson and Marit Nilsen-Hamilton 16. Immunohistochemical Methods Kendrick J. Morrell, Neil M. Hand, and Nicholas R. Griffin

AUTORE

Gary C. Howard, Ph.D.,is a senior scienfitic editor for the J. David Gladstone Institutes. Delia R. Bethell, Ph.D., is director of research with BioSeparations, Inc., in Tuscon, AZ.

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9780367200619
• Collana: Routledge Revivals
• Dimensioni: 9.25 x 6.25 in Ø 1.51 lb
• Formato: Copertina rigida
• Illustration Notes: 8 tables and 1 equations
• Pagine Arabe: 284