1. Genome Engineering of Yarrowia lipolytica with the piggyBac Transposon System
James M. Wagner, Claire M. Palmer, Maya V. Venkataraman, Lars H. Lauffer, Joshua M. Wiggers, Eden V. Williams, Xiunan Yi, and Hal S. Alper
2. Genome Editing in Y. lipolytica Using TALENs
Coraline Rigouin, Christian Croux, Gwendoline Dubois, Fayza Daboussi, and Florence Bordes
3. EasyCloneYALI: Toolbox for CRISPR-mediated Integrations and Deletions in Yarrowia lipolytica
Jonathan Dahlin, Carina Holkenbrink, and Irina Borodina
4. Simultaneous gene excision and integration by dual-guide CRISPR-Cas9
Michael Spagnuolo and Mark Blenner
5. Gene excision by dual-guide CRISPR-Cas9
Michael Spagnuolo and Mark Blenner
6. CRISPR Interference and Activation to Modulate Transcription in Yarrowia lipolytica
Joshua Misa and Cory Schwartz
7. Implementing CRISPR-Cas12a for Efficient Genome-editing in Yarrowia lipolytica
Zhiliang Yang and Peng Xu
8. Guide RNA Design for Genome-wide CRISPR Screens in Yarrowia lipolytica
Adithya Ramesh and Ian Wheeldon
9. Targeted Integration through Transformation of Hydroxyurea-Arrested Cells
Vasiliki Tsakraklides
10. Strain Construction for Intracellular Metabolic Pathway Localization in
Y. lipolytica Erin L. Bredeweg and Scott E. Baker
11. Title: Engineering Yarrowia lipolytica for production of fatty alcohols with YaliBrick Vectors
Wanqi Sun, Zhiliang Yang and Peng Xu
12. Methods to Activate and Elucidate Complex Endogenous Sugar Metabolism in Yarrowia lipolytica
Seunghyun Ryu and Cong T. Trinh
13. Pathway Engineering For Beta-Carotene and Carotenoid Biosynthesis in
Y. lipolytica Mauricio Pesantes-Munoz and Rodrigo Ledesma-Amaro
14. Droplet-based Microfluidic High-throughput Screening of Enzyme Mutant Libraries Secreted by Yarrowia lipolytica
Thomas Beneyton and Tristan Rossignol
15. Yarrowia lipolytica Bioprocess Development – From Flask to Bioreactor
Farshad Darvishi and Marzieh Moradi
16. Western Blotting of Membrane-Bound Proteins in
Yarrowia lipolytica Adithya Ramesh and Ian Wheeldon
