Xenotransplantation Methods and Protocols

This second edition volume delivers updated and new chapters on xenotransplantation covering a variety of methods. Despite many technological challenges faced by the xenotransplantation field, many major advances have been made in the last three decades. Expert researchers provide characterizations of xenogeneic interactions at the cellular and molecular levels, detailed descriptions of complex transplantation models, as well as attractive new tools for further advancement including 3D cell-culturing systems and genomic editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Thorough and intuitive, Xenotransplantation: Methods and Protocol, Second Edition aids scientists in continuing to study xenotransplantation and its multiple aspects.

1. Introduction: The Present Status of Xenotransplantation Research

David K.C. Cooper

 

2. Tools for Molecular Studies in Xenotransplantation

Sebastián G. Kuguel, Mireia Uribe-Herranz,  and Cristina Costa

 

3. Proteomic Protocols for Differential Protein Expression Analyses

Bent Honoré

 

4. Specific Detection of Neu5Gc in Animal Tissues by Immunohistochemistry

Anu Paul, Shirley Bachar Abramovitch, and Vered Padler-Karavani

 

5. Xenoantibodies and Complement Activity Determinations by Flow Cytometry in Pig-to-Primate Xenotrasplantation

Nieves Doménech and Pilar Sánchez-Corral

 

6. 3D Cell-Culture Models for the Assessment of Anticoagulant and Anti-inflammatory Properties of Endothelial Cells

Riccardo Sfriso and Robert Rieben

 

7. Cell-based Assays for Modeling Xenogeneic Immune Responses

Kelly Casós, Roberta Sommaggio, Magdiel Pérez-Cruz,  and Cristina Costa

 

8. Determination of Redox Status in Serum

 Kelly Casós, Cristina Costa, and Manuel Galiñanes

 

9. Generation of Genetically Modified Mice using CRISPR/Cas9

D. Muñoz-Santos, L. Montoliu and A. Fernández

 

10. Genome-wide PERV Inactivation in Pigs using CRISPR cas9

Marc Güell

 

11. Co-transplantation of Vascularized Thymic Graft with Kidney in Pig-to-Non-human Primates for the Induction of Tolerance Across Xenogeneic Barriers

Kazuhiko Yamada, Yuichi Ariyoshi, Thomas Pomposelli, and Mitsuhiro Sekijima

 

12. Xenogeneic Lung Transplantation Models

Lars Burdorf, Agnes M. Azimzadeh and Richard N. Pierson III

 

13. Liver Xenotransplantation in a Non-human Primate Model

Nalú Navarro-Alvarez and Parsia A. Vagefi

 

14. Intra-bone Bone Marrow Transplantation in Pig-to-Non-human Primates for the Induction of Tolerance Across Xenogeneic Barriers

Kazuhiko Yamada, Yuichi Ariyoshi, Thomas Pomposelli, and Kazuhiro Takeuchi

 

15. Studying Xenograft Rejection of Bioprosthetic Heart Valves

Rizwan A. Manji and Jacqueline S. Manji

 

16. Corneal Xenotransplantation: Anterior Lamellar Keratoplasty

Bertrand Vabres , Bernard Vanhove, and Gilles Blancho

 

17. Rat Model of Intra-articular Chondrocyte Xenotransplantation

Maribel Marquina, Magdiel Pérez-Cruz,  and Cristina Costa

 

18. Porcine Hepatocytes: Isolation and Liver Tissue Engineering for Xenotransplantation

Yi Li, Qiong Wu, Yujia Wang, Hong Bu, and Ji Bao

 

19. Pig-to-Macaque Islet Xenotransplantation

Suzanne Bertera, Michael F Knoll, Carmela A Knoll, David K Cooper, Massimo Trucco, and Rita Bottino

 

20. Regulation of Clinical Xenotransplantation: A Reappraisal of the Legal, Ethical and Social Aspects Involved

María Jorqui-Azofra