Urinary Biomarkers Methods and Protocols

This volume describes important methods, protocols, and techniques used for studying urinary biomarkers. Chapters detail different alterations use to studied different types of cancers and physiological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Urinary Biomarkers: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Part I: Urinary Biomarkers In Cancer

1. Urinary Biomarkers In Tumours: An Overview

2. Urinary Cell-free DNA Integrity Analysis

Valentina Casadio and Samanta Salvi

3. Mutational analysis in Urinary Cell-Free DNA: KRAS in Colorectal Cancer

4. Fluorescence In Situ Hybridization In Urine Samples (UroVysion Kit)

Sara Bravaccini

5. Analysis of Copy Number Variation In Urine: c-Myc Evaluation using a Real Time PCR Approach

6. Urinary microRNA and mRNA in Tumors

Erika Bandini

7. Long non-coding RNAs as Innovative Urinary Diagnostic Biomarkers

8. Urinary Nucleic Acid In Tumor: Bioinformatics Approaches

9. PCA3 in Prostate Cancer

Gunelli Roberta, Eugenia Fragalà, and Fiori Massimo

10. Urinary Exosomes in Prostate Cancer

Samanta Salvi, Erika Bandini, and Francesco Fabbri

11. Urinary Biomarkers In Bladder Cancer

Matteo Costantini, Graziana Gallo, and Giovanna Attolini

12. Telomerase Activity Analysis In Urine Sediment For Bladder Cancer

Valentina Casadio and Sara Bravaccini

13. Protocols for Preparation And Mass Spectrometry Analysis Of Clinical Urine Samples To Identify Candidate Biomarkers of Schistosoma-Associated Bladder Cancer

Tariq Ganief, Bridget Calder, and Jonathan M Blackburn

Part II: Urinary Extracellular Vesicles

14. Current Methods For The Isolation Of Urinary Extracellular Vesicles

Serena Maggio, Emanuela Polidori, Paola Ceccaroli, Andrea Cioccoloni, Vilberto Stocchi, and Michele Guescini

15. Urinary Extracellular Vesicles: Ultracentrifugation Method

Eisuke Tomiyama, Kazutoshi Fujita, and Norio Nonomura

Part III: Physical Activity and Urinary Markers

16. Urinary Catecholamines As Markers In Overtraining Syndrome

Leydi Natalia Vittori, Jenny Romasco, Andrea Tarozzi, and Pasqualino Maietta Latessa

Part IV: Urine Metabolic Markers

Serena De Matteis, Massimiliano Bonafè, and Anna Maria Giudetti