Tumor Angiogenesis Assays Methods and Protocols

This second edition provides new and updated methods and protocols for studying tumor angiogenesis in vitro and in vivo. Chapters detail morphological aspects of tumor angiogenesis, aortic ring, ex vivo tissue culture model for anti-angiogenic drug testing, transgenic zebrafish, orthotopic models of ovarian cancer, and uncovering metabolic effects of anti-angiogenic therapy in tumors by induced metabolic bioluminescence imaging.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Tumor Angiogenesis Assays: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about this important and developing field.

1. Discovery and development of tumor angiogenesis assays

Gianfranco Natale and Guido Bocci

 

2. The role of the stroma in tumor angiogenesis and progression: the fundamental contribution of Pietro M. Gullino

Domenico Ribatti

 

3.Methods for Isolation of Tumor Associated Endothelial Cells for Surface Protein Analysis and Sorting by Flow cytometry

Jelle Verhoeven, Patrizia Agostinis, and Madhur Agrawal

 

4. Perfusion based fluorescent dye labeling to sort cancer cells based on their distance from blood vessels

Saran Kumar

 

5. Visualizing the interactions that haping the imaging of microenvironment in cancer diseases

Antonio G. Solimando, Vanessa Desantis, and Matteo Claudio Da Vià

 

6. Quantification of tumor and angiogenic-related markers in ovarian cancer models by a digital pathology approach Ilaria Piga, Sonia Minuzzo, Tiziana Zanin, Giovanni Esposito, and Stefano Indraccolo

 

7. Monitoring angiotropic extravascular migratory metastasis in vitro

Claire Lugassy, Hynda K. Kleinman, and Raymond L. Barnhill

 

8. Double immunohistochemical staining on formalin-fixed paraffin-embedded tissue samples to study vascular co-option

Tiziana Annese, Mariella Errede, Michelina De Giorgis, Loredana Lorusso, Roberto Tamma, and Domenico Ribatti

 

9. Development of Experimental Three-Dimensional Tumor Models to Study Glioblastoma Cancer Stem Cells and Tumor Microenvironment

Henry Ruiz-Garcia, Natanael Zarco,  Fumihiro Watanabe,  Virginea De Araujo-Farias,  Paola Suarez-Meade,  Hugo Guerrero-Cazares, Jaime Imitola, Alfredo Quinones-Hinojosa, and  Daniel Trifiletti

 

10. IKOSA® CAM Assay application to quantify blood vessels on chick chorioallantoic membrane (CAM)

Tiziana Annese, Roberto Tamma, and Domenico Ribatti

 

11.Application of laser speckle contrast imaging (LSCI) for the angiogenesis measurement of tumors in the chorioallantoic membrane (CAM) model

Eric Pion, Silke Haerteis, and Thiha Aung

 

12. The chick CAM as an in vivo system to study stem cell activity

Marta Teixeira Pinto, Ana Sofia Ribeiro, and  Joana Paredes          

 

13. Detection of possible symmetries in vascular networks by computer assisted image analysis

Diego Guidolin, Cinzia Tortorella, abd  Domenico Ribatti

 

14. Alternative in vivo models to study teratoma

Michela Corsini and  Stefania Mitola

 

15. How to generate a vascular-labeled transgenic zebrafish model to study tumor angiogenesis and extravasation

Roxana E. Oberkersch, Jacopo Lidonnici, and Massimo M. Santoro

 

16. 3D human tumor tissues cultured in dynamic conditions as alternative in vitro disease models

Giorgia Pelizzoni and Silvia Scaglione