Preface…
Table of Contents…
Contributing Authors…
1. What a Difference 30 years Makes! A Perspective on Changes in research Methodologies used to Study Toxoplasma gondii
John C. Boothroyd
2. ToxoDB: Functional Genomics Resource for Toxoplasma and Related Organisms
Omar S. Harb and David S. Roos
3. Isolation and Genotyping of Toxoplasms gondii Strains
Chunlei Su and Jitender P. Dubey
4. Generation of Toxoplasma gondii and Hammondia hammondi Oocysts and Purification of their Sporozoites for Downstream Manipulation
Sarah L. Sokol, Sheen Wong, Jon P. Boyle, and Jitender P. Dubey
5. Assay for Monitoring Toxoplasms gondii Infectivity in the Laboratory Mouse
Qiuling Wang and L. David Sibley
6. PCR Screening of Toxoplasms gondii Single Clones Directly from 96-Well Plantes without DNA Purification
Federica Piro, Vern B. Carruthers, and Manlio Di Cristina
7. CRISPR/Cas9-Mediated Generation of Tetracycline Repressor-Based Inducible Knock-Down in Toxoplasma gondii
Damien Jacot and Dominique Soldati-Favre
8. Assessing Rhyptry Secretion in T. gondii
Catherine Suarez, Melissa B. Lodoen, and Maryse Lebrun
9. High-Throughput Measurement of Microneme Secretion in Toxoplasma gondii
Kevin M. Brown, L. David Sibley, and Sebastian Lourido
10. Plate-Based Quantification of Stimulated Toxoplasma egress
Emily Shortt and Sebastian Lourido
11. Genetic Indicators for Calcium Signaling Studies in Toxoplasma gondii
Stephen A. Vella, Abigail Calixto, Beejan Asady, Zhu-Hong Li, and Silvia N.J. Moreno
12. Phenotyping Toxoplasma Invasive Skills by Fast Live Cell Imaging
Georgios Pavlou and Isabelle Tardieux
13. Experimental Approaches for Examining Apicoplast Biology
Marco Biddau, Jana Ovciarikova, and Lilach Sheiner
14. Measuring Solute Transport in Toxoplasma gondii Parasites
Esther Rajendran, Kiaran Kirk, and Giel G. van Dooren
15. Toxoplasma gondii: Bradyzoite Differentiation In Vitro and In Vivo
Joshua Mayoral, Manlio Di Cristina, Vern B. Carruthers, and Louis M. Weiss
16. Three-Dimensional Reconstruction of Toxoplasma-Neuron Interactions In Situ
Carla M. Cabral, Emily F. Merritt, and Anita A. Koshy
17. Model Systems for Studying Mechanisms of Ocular Toxoplasmosis
Justine R. Smith, Liam M. Ashander, Yuefang Ma, Elise Rochet, and João M. Furtado
18. Using BioID for the Identification of Interacting and Proximal Proteins in Subcellular Compartments in Toxoplasma gondii
Peter J. Bradley, Shima Rayatpisheh, James A. Wohlschlegel, and Santhosh M. Nadipuram
19. Assays to Evaluate Toxoplasma-Macrophage Interactions
Debanjan Mukhopadhyay and Jeroen P.J. Saeij
20. Methods for the Measurement of Early Events in Toxoplasma gondii Immunity in Mouse Cells
Catalina Alvarez, Ana Claudia Campos, Jonathan C. Howard, Joana Loureiro, Urs Benedikt Müller, and Ana Lina Rodrigues
21. Image-based Quantitation of Host Cell-Toxoplasma gondii Interplay using HRMan; A Host Response to Microbe Analysis Pipeline
Daniel Fisch, Artur Yakimovich, Barbara Clough, Jason Mercer, and Eva-Maria Frickel
22. Metabolomic Analysis of Toxoplasma gondii Tachyzoites
Elizabeth King, Simon Cobbold, Alessandro D. Uboldi, Christopher J. Tonkin, and Malcolm J. McConville
23. Label-Based Mass Spectrometry Approaches for Robust Quantification of the Phospho- and Total Proteome in Toxoplasma gondii
Malgorzata Broncel and Moritz Treeck
