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Therapeutic Proteins Methods and Protocols

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Dettagli

Genere:Libro
Lingua: Inglese
Editore:

Humana

Pubblicazione: 06/2012
Edizione: 2nd ed. 2012





Trama

After the identification of a potential protein drug, the next critical step is the production of sufficient authentic material for testing, characterization, and clinical trials, which, when successful, leads to the need for robust methodologies for large-scale production, purification, characterization, viral inactivation, and continued testing of the final protein product.  Building on the valuable first edition, Therapeutic Proteins: Methods and Protocols, Second Edition aims to cover each of these key aspects of protein drug production through the contributions of authors from highly esteemed industrial and academic institutions around the world.  Emphasizing the newest developments in the field, this second edition also includes additional emphasis on discovery, including new display and screening methods as well as the design and engineering of new types of therapeutic proteins.  There is also discussion of computational and bioinformatics methods, and chapters on safety aspects of therapeutic protein development.  Written in the highly successful Methods in Molecular Biology™ format, protocol chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Fully updated and practical, Therapeutic Proteins: Methods and Protocols, Second Edition provides an essential resource to all scientists working in the field of therapeutic proteins.




Sommario

1. Therapeutic Proteins

            Dimiter S. Dimitrov

2. Synthetic Antibody Libraries

            Bryce Nelson and Sachdev S. Sidhu

3. The Construction of ‘Phylomer’ Peptide Libraries as a Rich Source of Potent Inhibitors of Protein/Protein Interactions

            Nadia Milech and Paul Watt

4. Ribosome Display and Screening for Protein Therapeutics

            Damjana Kastelic and Mingyue He

5. Yeast Display of Engineered Antibody Domains

            Qi Zhao, Zhongyu Zhu, and Dimiter S. Dimitrov

6. Design, Expression, Purification, and Characterization of Engineered Antibody CH2 and VH Domains

            Rui Gong, Weizao Chen, and Dimiter S. Dimitrov

7. Engineering of Affibody Molecules for Therapy and Diagnostics

            Joachim Feldwisch and Vladimir Tolmachev

8. Protein Design for Diversity of Sequences and Conformations Using Dead-End Elimination

            Karl J.M. Hanf

9. Design and Generation of DVD-IgTM Molecules for Dual-Specific Targeting

            Enrico DiGiammarino, Tariq Ghayur, and Junjian Liu

10. Engineering and Expression of Bibody and Tribody Constructs in Mammalian Cells and in the Yeast Pichia pastoris

            Steve Schoonooghe

11. Use of E. coli for the Production of a Single Protein

            Lili Mao and Masayori Inouye

12. Folding Engineering Strategies for Efficient Membrane Protein Production in E. coli

            Brent L. Nannenga and François Baneyx

 

13. Transient Expression Technologies: Past, Present, and Future

            Sabine Geisse and Bernd Voedisch

14. Stable Transfection Pools for Large Quantity of Protein Production

            Jianxin Ye

15. Mammalian Stable Expression of Biotherapeutics

            Thomas Jostock and Hans-Peter Knopf

16. Transgenic Expression of Therapeutic Proteins in Arabidopsis thaliana Seed

            Cory L. Nykiforuk and Joseph G. Boothe

17. Methods for Chromatographic Removal of Endotoxin

            Adam J. Lowe, Cameron L. Bardliving, and Carl A. Batt

18. Effectiveness of Various Processing Steps for Viral Clearance of Therapeutic Proteins: Database Analyses of Commonly-Used Steps

            Dana Cipriano, Michael Burnham, and Joseph V. Hughes

19. High-Throughput Quantitative N-Glycan Analysis of Glycoproteins

            Margaret Doherty, Ciara A. McManus, Rebecca Duke, and Pauline M. Rudd

20. High-Throughput Multimodal Strong Anion Exchange Purification and N-Glycan Characterization of Endogenous Glycoprotein Expressed in Glycoengineered Pichia pastoris

            Sujatha Gomathinayagam, Erik Hoyt, Alissa M. Thompson, Eric Brown, Khanita Karaveg, Stephen Hamilton, and Huijuan Li

21. Databases and Tools in Glycobiology

            Natalia V. Artemenko, Andrew G. McDonald, Gavin P. Davey, and Pauline M. Rudd

22. Characterization of PEGylated Biopharmaceutical Products by LC/MS and LC/MS/MS

            Lihua Huang and P. Clayton Gough

23. Identification of Asp Isomerization in Proteins by 18O Labeling and Tandem Mass Spectrometry

            Jennifer Zhang and Viswanatham Katta

24. Monitoring of Subvisible Particles in Therapeutic Proteins

            Satish K. Singh and Maria R. Toler

25. Size-Exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS) for Elucidating Protein Aggregation Mechanisms

            Erinc Sahin and Christopher J. Roberts

26. Computational Methods to Predict Therapeutic Protein Aggregation

            Patrick M. Buck, Sandeep Kumar, Xiaoling Wang, Neeraj J. Agrawal, Bernhardt L. Trout, and Satish K. Singh

27. Coarse-Grained Simulations of Protein Aggregation

            Troy Cellmer and Nicolas L. Fawzi

28. Chitosan-Based Nanoparticles as Delivery Systems of Therapeutic Proteins

            Pedro Fonte, José Carlos Andrade, Vítor Seabra, and Bruno Sarmento

29. Challenges in the Development and Manufacturing of Antibody-Drug Conjugates

            Laurent Ducry

            Lihua Huang and P. Clayton Gough











Altre Informazioni

ISBN:

9781617799204

Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 254 x 178 mm Ø 1157 gr
Formato: Copertina rigida
Illustration Notes:XI, 502 p.
Pagine Arabe: 502
Pagine Romane: xi


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