Single Molecule Microscopy In Neurobiology - Yamamoto Nobuhiko (Curatore); Okada Yasushi (Curatore) | Libro Humana 04/2021 -

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yamamoto nobuhiko (curatore); okada yasushi (curatore) - single molecule microscopy in neurobiology

Single Molecule Microscopy in Neurobiology


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Lingua: Inglese


Pubblicazione: 04/2021
Edizione: 1st ed. 2020


This volume looks at the methodology and techniques used by experts to study how certain molecules function in specific locations, and their temporal patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by quantum-dot single particle tracking; SPoD-OnSPAN; and investigating molecular diffusion inside small neuronal compartments with two-photon fluorescence correlation spectroscopy. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and comprehensive, Single Molecule Microscopy is a valuable resource for any researcher interested in learning more about this important field. 


Series Preface…
Table of Contents…
Contributing Authors…

1. Single Molecule Imaging of Intracellular Transport in Neurons and Non-Neuronal Cells: From Microscope Optics to Sample Preparations
Jay Wang, Taketoshi Kambara, and Yasushi Okada

2. In Vivo Single-Molecule Tracking of Voltage-Gated Calcium Channels with Split-Fluorescent Proteins in CRISPR-Engineered C. elegans
Yunke Zhao and Fabien Pinaud

3. Nanocores and Liquid Droplets: Single-Molecule Microscopy of Neuronal Stress Granule Components
Benedikt Niewidok, Rainer Kurre, and Roland Brandt

4. Live-Cell Single-Molecule Imaging with Optogenetics Reveals Dynamics of an Activity-Dependent Transcription Factor
Hironobu Kitagawa, Noriyuki Sugo, and Nobuhiko Yamamoto

5. Single-Molecule Imaging of Recycling Synaptic Vesicles in Live Neurons
Merja Joensuu, Ramon Martínez-Mármol, Mahdie Mollazade, Pranesh Padmanabhan, and Frédéric A. Meunier

6. Approaching Protein-Protein Interactions in Membranes using Single Particle Tracking and Packing Coefficient Analysis
Marianne Renner and Antoine Triller

7. Synaptic Function and Neuropathological Disease Revealed by Quantum-Dot Single Particle Tracking
Hiroko Bannai, Takafumi Inoue, Matsumi Hirose, Fumihiro Niwa, and Katsuhiko Mikoshiba

8. Multipolarization Darkfield Imaging of Single Endosomes in Microfluidic Neuronal Culture for Simultaneous Orientation and Displacement Tracking
Luke Kaplan and Bianxiao Cui

9. Practical Guidelines for Two-Color SMLM of Synaptic Proteins in Cultured Neurons
Xiaojuan Yang and Christian G. Specht

10. Single-Molecule Localization Microscopy Propelled by Small Organic Fluorophores with Blinking Properties
Akihiko Morozumi, Mako Kamiya, and Yasuteru Urano

11. Highly-Biocompatible Super-Resolution Imaging: SPoD-OnSPAN
Tetsuichi Wazawa, Takashi Washio, and Takeharu Nagai

12. Nanoscale Molecular Imaging of Presynaptic Active Zone Proteins in Cultured Hippocampal Neurons
Hirokazu Sakamoto, Shigeyuki Namiki, and Kenzo Hirose

13. Three Dimensional Super-Resolution Imaging of the Cytoskeleton in Hippocampal Neurons using Selective Plane Illumination
Frances Camille M. Wu, Feby Wijaya Pratiwi, Chin-Yi Chen, Chieh-Han Lu, Wei-Chun Tang, Yen-Ting Liu, Bi-Chang Chen, and Peilin Chen

14. A Protocol for Single Molecule Translation Imaging in Xenopus Retinal Ganglion Cells
Florian Ströhl, Julie Qiaojin Lin, Francesca W. van Tartwijk, Hovy Ho-Wai Wong, Christine E. Holt, and Clemens F. Kaminski

15. Investigating Molecular Diffusion Inside Small Neuronal Compartments with Two-Photon Fluorescence Correlation Spectroscopy
Kazuki Obashi and Shigeo Okabe

Altre Informazioni



Condizione: Nuovo
Collana: Neuromethods
Dimensioni: 254 x 178 mm Ø 662 gr
Formato: Brossura
Illustration Notes:21 Illustrations, black and white
Pagine Arabe: 331
Pagine Romane: xiii

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