Single Molecule Microscopy in Neurobiology

This volume looks at the methodology and techniques used by experts to study how certain molecules function in specific locations, and their temporal patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by quantum-dot single particle tracking; SPoD-OnSPAN; and investigating molecular diffusion inside small neuronal compartments with two-photon fluorescence correlation spectroscopy. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and comprehensive, Single Molecule Microscopy is a valuable resource for any researcher interested in learning more about this important field. 

Series Preface…

Preface…

Table of Contents…

Contributing Authors…

1. Single Molecule Imaging of Intracellular Transport in Neurons and Non-Neuronal Cells: From Microscope Optics to Sample Preparations

Jay Wang, Taketoshi Kambara, and Yasushi Okada

2. In Vivo Single-Molecule Tracking of Voltage-Gated Calcium Channels with Split-Fluorescent Proteins in CRISPR-Engineered C. elegans

Yunke Zhao and Fabien Pinaud

3. Nanocores and Liquid Droplets: Single-Molecule Microscopy of Neuronal Stress Granule Components

Benedikt Niewidok, Rainer Kurre, and Roland Brandt

4. Live-Cell Single-Molecule Imaging with Optogenetics Reveals Dynamics of an Activity-Dependent Transcription Factor

Hironobu Kitagawa, Noriyuki Sugo, and Nobuhiko Yamamoto

5. Single-Molecule Imaging of Recycling Synaptic Vesicles in Live Neurons

Merja Joensuu, Ramon Martínez-Mármol, Mahdie Mollazade, Pranesh Padmanabhan, and Frédéric A. Meunier

6. Approaching Protein-Protein Interactions in Membranes using Single Particle Tracking and Packing Coefficient Analysis

Marianne Renner and Antoine Triller

7. Synaptic Function and Neuropathological Disease Revealed by Quantum-Dot Single Particle Tracking

Hiroko Bannai, Takafumi Inoue, Matsumi Hirose, Fumihiro Niwa, and Katsuhiko Mikoshiba

8. Multipolarization Darkfield Imaging of Single Endosomes in Microfluidic Neuronal Culture for Simultaneous Orientation and Displacement Tracking

Luke Kaplan and Bianxiao Cui

9. Practical Guidelines for Two-Color SMLM of Synaptic Proteins in Cultured Neurons

Xiaojuan Yang and Christian G. Specht

10. Single-Molecule Localization Microscopy Propelled by Small Organic Fluorophores with Blinking Properties

Akihiko Morozumi, Mako Kamiya, and Yasuteru Urano

11. Highly-Biocompatible Super-Resolution Imaging: SPoD-OnSPAN

Tetsuichi Wazawa, Takashi Washio, and Takeharu Nagai

Hirokazu Sakamoto, Shigeyuki Namiki, and Kenzo Hirose

13. Three Dimensional Super-Resolution Imaging of the Cytoskeleton in Hippocampal Neurons using Selective Plane Illumination

Frances Camille M. Wu, Feby Wijaya Pratiwi, Chin-Yi Chen, Chieh-Han Lu, Wei-Chun Tang, Yen-Ting Liu, Bi-Chang Chen, and Peilin Chen

14. A Protocol for Single Molecule Translation Imaging in Xenopus Retinal Ganglion Cells

Florian Ströhl, Julie Qiaojin Lin, Francesca W. van Tartwijk, Hovy Ho-Wai Wong, Christine E. Holt, and Clemens F. Kaminski

15. Investigating Molecular Diffusion Inside Small Neuronal Compartments with Two-Photon Fluorescence Correlation Spectroscopy

Kazuki Obashi and Shigeo Okabe