Rna Remodeling Proteins - Boudvillain Marc (Curatore) | Libro Humana 11/2020 - HOEPLI.it


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RNA Remodeling Proteins Methods and Protocols




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Dettagli

Genere:Libro
Lingua: Inglese
Editore:

Humana

Pubblicazione: 11/2020
Edizione: 2nd ed. 2021





Sommario

1. Key Points to Consider when Studying RNA Remodeling by Proteins

W. Luke Ward and Rick Russell

 

2. Happy Birthday : 30 Years of RNA Helicases

Martina Valentini and Patrick Linder

 

3. Known Inhibitors of RNA Helicases and their Therapeutic Potential

Yosser Zina Abdelkrim, Josette Banroque, and  N. Kyle Tanner

 

4. Identifying RNA Helicase Inhibitors Using Duplex Unwinding Assays

John C. Marecki, Alicia K. Byrd, and Kevin D. Raney

 

5. Thermal Shift Assay for Characterizing the Stability of RNA Helicases and their Interaction with Ligands

Emmanuel Saridakis and Franck Coste

 

6. SRCD and FTIR Spectroscopies to Monitor Protein-induced Nucleic Acid Remodeling

Frank Wien, Frédéric Geinguenaud, Wilfried Grange, and Véronique Arluison

 

7. High throughput Protein-Nucleic Acid Interaction Assay Based on Protein Induced Fluorescence Enhancement

Jaroslav Fulnecek and Karel Ríha

 

8. Probing RNA Helicase Conformational Changes by Single-molecule FRET Microscopy

Linda Krause and Dagmar Klostermeier

 

9. A Fluorescent Assay to Monitor Ligand-Dependent Closure of the Hexameric Rho Helicase Ring

Michael R. Lawson and James M. Berger

 

10. A Simple Fluorescence Microplate Assay to Monitor RNA-DNA Hybrid Unwinding by the Bacterial Transcription Termination Factor Rho

Isabelle Simon and Marc Boudvillain

 

11. Monitoring Enzymatic RNA G-quadruplex Unwinding Activities by Nuclease Sensitivity and Reverse Transcription Stop Assays

Ewan K.S. McRae, Steven J. Dupas, Negar Atefi,  and Sean A. McKenna

 

12. Using Magnetic Tweezers to Unravel the Mechanism of the G-quadruplex Binding and Unwinding Activities of DHX36 Helicase

Huijuan You, Yu Zhou, and Jie Yan

 

13. Characterization of the Brr2 RNA Helicase and its Regulation by other Spliceosomal Proteins using Gel-based U4/U6 di-snRNA Binding and Unwinding Assays

Eva Absmeier and Markus C. Wahl

 

14. Monitoring Acetylation of the RNA-helicase DDX3X, a Protein Critical for Formation of Stress Granules

Makoto Saito, Vytautas Iestamantavicius, Daniel Hess, and Patrick Matthias

 

15. In vivo cross-linking Followed by polyA Enrichment to Identify Yeast mRNA Binding Proteins

Sarah F. Mitchell

 

16. Deciphering the Dynamic Landscape of Transcription-associated mRNP quality Control Components over the Whole Yeast Genome

Kévin Moreau, Aurélia Le Dantec, and A. Rachid Rahmouni

 

17. Probing the Conformational State of mRNPs using smFISH and SIM

Srivathsan Adivarahan and Daniel Zenklusen

 

18. Probing Transcriptome-wide RNA Structural Changes Dependent on the DEAD-box Helicase Dbp2

Yu-Hsuan Lai and Elizabeth J. Tran

 

19. In situ hybridization – Proximity Ligation Assay (ISH-PLA) to Study the Interaction of HIV-1 RNA and Remodeling Proteins

Daniela Toro-Ascuy, Aracelly Gaete-Argel, Victoria Rojas-Celis, and Fernando Valiente-Echeverria

 

20. RNAi Screening to Identify Factors that Control Circular RNA Localization

Deirdre C. Tatomer, Dongming Liang, and Jeremy E. Wilusz

 

21. A Cell-Free System for Investigating Human MARF1 Endonuclease Activity

Hana Fakim and Marc R. Fabian

 

22. Monitoring eIF4E-dependent Nuclear 3’ end mRNA Cleavage

Mildred Delaleau

 

23. An In Vitro Approach To Study RNase III Activities Of Plant RTL Proteins Cyril Charbonnel, Anne de Bures, and Julio Sáez-Vásquez

 

24. Analysis of Bacillus subtilis Ribonuclease Activity In Vivo

Laetitia Gilet, Olivier Pellegrini, Aude Trinquier, Anastasia Tolcan, Delphine Allouche, Frédérique Braun, Sylvain Durand, and Ciarán Condon

 

25. Assay of Bacillus subtilis Ribonuclease Activity in vitro

Olivier Pellegrini, Laetitia Gilet, Aude Trinquier, Anastasia Tolcan, Delphine Allouche, Sylvain Durand, Frédérique Braun, and Ciarán Condon

 

26. Protein Pulldown Assays to Monitor the Composition of the Bacterial RNA Degradosome

Steven W. Hardwick, Ben F. Luisi, and Marilis V. Marques

 

 

 

 

 

 

 





Trama

This volume provides the most current methods to study RNA remodeling proteins. Chapters detail methods, ranging from basic to complex, procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex molecular mechanisms of action using purified components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, RNA Remodeling Proteins: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.








Altre Informazioni

ISBN:

9781071609347

Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 254 x 178 mm Ø 1041 gr
Formato: Copertina rigida
Illustration Notes:20 Illustrations, black and white
Pagine Arabe: 437
Pagine Romane: xiv






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