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schillberg stefan (curatore); spiegel holger (curatore) - recombinant proteins in plants

Recombinant Proteins in Plants Methods and Protocols


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Lingua: Inglese


Pubblicazione: 05/2022
Edizione: 1st ed. 2022


This volume provided methods and protocols on recombinant protein production in different plant systems, downstream processing, and strategies to optimize protein expression. Chapters guide readers through recombinant protein production in important plant systems, protein recovery and purification, different strategies to optimise productivity, cloning and fusion protein approaches, and the regulation and freedom to operate analysis of plant-produced proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Recombinant Proteins in Plants: Methods and Protocols aims to be useful to newcomers and experienced researchers interested in expanding their expertise in the field of plant-based protein production.

Chapters 6, 8 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.


1. Recombinant Protein Production in Plants – A Brief Overview of Strengths and Challenges

Stefan Schillberg and Holger Spiegel


Part I: Plant Production Systems and Applications


2. Production of Recombinant Proteins in Transgenic Tobacco Plants

Tim H. Szeto, Pascal M.W. Drake, Audrey Y.-H. Teh, Nicole Falci Finardi, Ashleigh G. Clegg, Mathew J. Paul, Rajko Reljic, and Julian K.-C. Ma


3. Molecular Farming in Seed Crops: Gene Transfer into Barley (Hordeum vulgare) and wheat (Triticum aestivum)

Eszter Kapusi and Eva Stoger


4. Cell Biology Methods to Study Recombinant Proteins in Seeds

Elsa Arcalís, Emanuela Pedrazzini, Ulrike Hörmann-Dietrich, Alessandro Vitale, and Eva Stoger


5. Production of Recombinant Glycoproteins in Nicotiana tabacum BY-2 Suspension Cells

Catherine Navarre and François Chaumont


6. Production of Recombinant Proteins by Agrobacterium-mediated Transient Expression

Holger Spiegel, Stefan Schillberg, and Greta Nölke


7. Specific packaging of custom RNA molecules into Cowpea Mosaic Virus-Like Particles

Hadrien Peyret and George P. Lomonossoff


8. Plant-based Cell-Free In Vitro Transcription and Translation of Recombinant Proteins

Matthias Buntru, Simon Vogel, Ricarda Finnern, and Stefan Schillberg



Part II: Downstream Processing


9. Strategies for Efficient and Sustainable Protein Extraction and Purification from Plant Tissues

Johannes F. Buyel


10. Improving Recombinant Protein Recovery from Plant Tissue Using Heat Precipitation

Holger Spiegel


11. Techno-economic Modeling and Simulation for Plant-Based Manufacturing of Recombinant Proteins

Matthew J. McNulty, Somen Nandi, and Karen A. McDonald


Part III: Optimization Strategies


12. Optimization of Vectors and Targeting Strategies Including Goldenbraid and Genome Editing Tools -

GoldenBraid assembly of multiplex CRISPR/Cas12a guide RNAs for Gene Editing in Nicotiana benthamiana

Beatriz González, Marta Vazquez-Vilar, Javier Sánchez-Vicente, and Diego Orzáez


13. Advanced Fusion Strategies for The Production Of Functionalized Potato Virus X virions

Christina Dickmeis and Ulrich Commandeur


14. Knockout of Glycosyltransferases in Nicotiana benthamiana by Genome Editing to Improve Glycosylation of Plant-Produced Proteins

Julia Jansing and Luisa Bortesi


15. A bioluminescent Agrobacterium tumefaciens for Imaging Bacterial Metabolic Activity in planta

Philippe V. Jutras, Isobel Dodds, and Renier A. L. van der Hoorn


16. Statistical Designs to Improve Downstream Processing

Johannes F. Buyel


 Part IV: Regulatory Issues

 17. Regulation of Molecular Farming Products

Penny AC Hundleby, Marc-André D’Aoust, Carolyn Finkle, Judith Atkins, and Richard M. Twyman


18. Freedom to Operate Analysis of Molecular Farming Projects

Harry Thangaraj

Altre Informazioni



Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 254 x 178 mm Ø 867 gr
Formato: Copertina rigida
Illustration Notes:X, 346 p. 51 illus., 39 illus. in color.
Pagine Arabe: 346
Pagine Romane: x

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