Receptor and Ion Channel Detection in the Brain

This second edition volume expands on the previous edition with new information on the latest developments in the field, and the most up-to-date representative methodological information on molecular neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters in this book are organized into three parts. Part One discusses molecular immunological and pharmacological aspects related to the detection of receptors and ion channels in the brain. Part Two explores the neuroanatomical techniques devoted to localizing receptors and ion channels within the central nervous system. Part Three looks at functional approaches devoted to ascertaining receptors and ion channels functionality in the brain. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and comprehensive, Receptor and Ion Channels Detection in the Brain, Second Edition is a valuable resource that contains detailed molecular, cellular, neuroanatomical, and electrophysiological methods that will help researchers study neurotransmitter receptor and ion channel in any region of neuron of the brain.

Production of High-Quality Antibodies for the Study of Receptors and Ion Channels.- Co-Immunoprecipitation from Brain.- Sub-Synaptic Membrane Fractionation.- Quantitative Analysis of Cell Surface Expressed, Intracellular, and Internalized Neurotransmitter Receptor Populations in Brain Slices Using Biotinylation.- Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells.- Radioligand Binding Detection of Receptors in Brain Membranes.- Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain.- Time Resolved-Fluorescence Resonance Energy Transfer using Fluorescent Ligands to Study Native G Protein-Coupled Receptor Heteromerization in Brain.- Study of GPCR Homo and Heteroreceptor Complexes in Specific Neuronal Cell Populations using the In Situ Proximity Ligation Assay.- Amplified Luminescent Proximity Homogeneous Assay (Alpha)-Based Technique to Detect GPCR Oligomers in Human Post-Mortem Brain.- Fluorescent In Situ Hybridization for Sensitive and Specific Labeling.- Autoradiographic Visualization of G Protein-Coupled Receptors in Brain.- Localization of Neurotransmitter Receptor and Ion Channel Proteins in Unfixed Brains using In Situ Immunoblotting.- Immunohistochemistry for Ion Channels and their Interacting Molecules: Tips for Improving Antibody Accessibility.- Localization of GFP-Tagged Proteins at the Electron Microscope.- Tyramide Signal Amplificación for Immunoelectron Microscopy.- Pre-Embedding Methods for the Localization of Receptors and Ion Channels.- Post-Embedding Immunohistochemistry in the Localization of Receptors and Ion Channels.- High-Resolution Localization and Quantitation of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL).- Pre-Embedding Immunostaining of Brain Tissue and Three-Dimensional Imaging with FIB-SEM.- Application of Virus Vectors for Anterograde Tract-Tracing and Single-Neuron Labeling Studies.- Efficient Labeling of Neurons and Identification of Postsynaptic Sites Using Adeno-Associated Virus Vector.- Analysis of Synaptic Connections at the Electron Microscopic Level using Sindbis Virus Vectors.- Morphological and Neurochemical Characterization of Electrophysiologically Identified Cells.- Using Electrophysiology to Study Synaptic and Extrasynaptic Ionotropic Receptors in Hippocampal Neurons.- Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium Channels.- Electrophysiological Recordings in Behaving Animals.- Voltammetry in Behaving Animals.- In Vivo Brain Microdialysis of Monoamines.- Optical Control of Brain Receptors using Photoactive Drug in Behaving Animals.- Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor.- Monitoring GPCR-Mediated cAMP Accumulation in Rat Striatal Synaptosomes.- GPCR-Mediated MAPK/ERK Cascade Activation in Mouse Striatal Slices.