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Reactive Oxygen Species in Plants Methods and Protocols

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Lingua: Inglese


Pubblicazione: 06/2022
Edizione: 1st ed. 2022


This detailed volume explores techniques to study reactive oxygen species (ROS) in plants and to characterize their roles in development and stress responses. Beginning with a section on strategies to induce ROS production, the book continues with methods to visualize ROS and detect changes in redox homeostasis, small-scale and targeted analyses for investigating the effects of ROS accumulation during stress on plant physiology and metabolism, as well as systems biology approaches to understand ROS functions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Reactive Oxygen Species in Plants: Methods and Protocols serves as a vital resource that any researcher, and in particular young researchers, can use and adapt to further our knowledge of this dynamic area of plant science.


Part I: Strategies to Induce ROS Production


1. Modification of Chloroplast Antioxidant Capacity by Plastid Transformation

            Shengchun Li, Pan Shen, Bipeng Wang, Xiujie Mu, Mimi Tian, Tao Chen, and Yi Han


2. Analysis of Ascorbate Metabolism in Arabidopsis under High-Light-Stress

            Takanori Maruta and Takahiro Ishikawa


3. Genetic Manipulation of Reactive Oxygen Species (ROS) Homeostasis Utilizing CRISPR/Cas9-Based Gene Editing in Rice

            Sheng Xu, Tao Chen, Mimi Tian, Marie-Sylviane Rahantaniaina, Linlin Zhang, Ren Wang, Wei Xuan, and Yi Han


4. Studying Plant Stress Reactions In Vivo by PAM Chlorophyll Fluorescence Imaging

            Alexey Shapiguzov and Jaakko Kangasjärvi


Part II: Methods to Visualize ROS and to Detect Changes in Redox Homeostasis


5. Live Monitoring of ROS-Induced Cytosolic Redox Changes with roGFP2-Based Sensors in Plants

            José Manuel Ugalde, Lara Fecker, Markus Schwarzländer, Stefanie J. Müller-Schüssele, and Andreas J. Meyer


6. Quantitative Measurement of Ascorbate and Glutathione by Spectrophotometry

            Graham Noctor and Amna Mhamdi


7. Measurement of NAD(P)H and NADPH-Generating Enzymes

            Amna Mhamdi, Frank Van Breusegem, and Graham Noctor


8. Quantitative Analysis for ROS-Producing Activity and Regulation of Plant NADPH Oxidases in HEK293T Cells

            Sachie Kimura, Hidetaka Kaya, Kenji Hashimoto, Michael Wrzaczek, and Kazuyuki Kuchitsu


Part III: Small-Scale Targeted Analysis of ROS Accumulation during Stress and Effects on Plant Physiology


9. Estimation of the Level of Abasic Sites in Plant mRNA Using Aldehyde Reactive Probe

            Jagna Chmielowska-Bak, Karolina Izbianska-Jankowska, and Joanna Deckert


10. A Simplified Method to Assay Protein Carbonylation by Spectrophotometry

            Corentin Moreau and Emmanuelle Issakidis-Bourguet


11. In Vitro Biochemical Analysis of Recombinant Plant Proteins under Oxidation

            Zeya Chen and Jingjing Huang


12. Methods to Analyze the Redox Reactivity of Plant Proteins

            Thualfeqar Al-Mohanna, George V. Popescu, and Sorina C. Popescu


13. Determination of ROS-Induced Lipid Peroxidation by HPLC-Based Quantification of Hydroxy Polyunsaturated Fatty Acids

            Brigitte Ksas and Michel Havaux


14. Detection of Lipid Peroxidation-Derived Free Azelaic Acid, a Biotic Stress Marker and Other Dicarboxylic Acids in Tobacco by Reversed Phase HPLC-MS under Non-Derivatized Conditions

            Attila L. Ádám, György Kátay, András Künstler, and Lóránt Király


15. Determination of Reactive Carbonyl Species, which Mediate Reactive Oxygen Species Signals in Plant Cells

            Jun'ichi Mano, Md. Sanaullah Biswas, Koichi Sugimoto, and Yoshiyuki Murata


16. Measuring Stress-Induced Changes in Defense Phytohormones and Related Compounds

            Caroline Lelarge-Trouverie, Amna Mhamdi, Florence Guérard, and Graham Noctor


Part IV: Systems Biology Approaches to Understand ROS Functions


17. Targeted Mass Spectrometry Analysis of Protein Phosphorylation by Selected Ion Monitoring Coupled to Parallel Reaction Monitoring (tSIM/PRM)

            Jesús Pascual and Saijaliisa Kangasjärvi


18. Quantitative Analysis of Post-Translational Modifications of Plant Histones

            Hana Kucharíková, Zuzana Plšková, Zbynek Zdráhal, Miloslava Fojtová, Pavel Kerchev, and Gabriela Lochmanová


19. Characterization of RBPome in Oxidative Stress Conditions

            Zhicheng Zhang, Evy Timmerman, Francis Impens, and Frank Van Breusegem


20. Analysis of ROS-Triggered Changes in the Transcriptome

            Patrick Willems

Altre Informazioni



Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 254 x 178 mm Ø 787 gr
Formato: Copertina rigida
Illustration Notes:XIV, 298 p. 53 illus., 36 illus. in color. With online files/update.
Pagine Arabe: 298
Pagine Romane: xiv

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