This book covers up-to-date evidence of Rapid on-site evaluation (ROSE) for diagnosis of rare disease and used combined with metagenomic sequencing technique in clinical scenarios. ROSE has becoming an efficient interventional diagnostics tool and important reference for lung diseases differentiation, increasing the adequacy rate, diagnostic yield and accuracy of the interventional pulmonary procedures.ROSE has its value in the field of evaluating sampling adequacy for further immunohistochemistry, microbiology studies, flow cytometry analysis, and molecular assays; DNA analysis of biopsy specimens using sequencing technique is performed to provide clue of etiology, which is an emerging pathway for clinical dilemmas. The first part of the book discusses the principals of performing ROSE procedures and reviews the basic concepts and summarized knowledge of key anatomical structures in lung. Figures of ROSE cytopathology, together with clinical data, results of metagenomic sequencing technique are presented to demonstrate how the difficulties in clinical practice could be tackled with the help of advanced techniques. Further application direction and perspective are also discussed, which provides researchers with more insights on the discipline and techniques.
This book is part of the 4-volume collection ROSE in Diagnostic Interventional Pulmonology.
Rapid on-site evaluation (ROSE) in diagnostic interventional pulmonology.- Anatomic distribution and morphology of common tracheal/bronchial/pulmonary cells.- Clinical data, ROSE cytopathology and metagnomic sequencing analysis in diagnosis of bacterial infection.- Clinical data, ROSE cytopathology and metagnomic sequencing analysis in diagnosis of viral infection.- Clinical data, ROSE cytopathology and metagnomic sequencing analysis in diagnosis of fungal infection.- Clinical data, ROSE cytopathology and metagnomic sequencing analysis in diagnosis of mixed infection.- Limitations and future direction.
Jing Feng is a professor and chief at the Division of Pulmonary and Critical Care Medicine and Department of Interventional Pulmonology, Tianjin Medical University General Hospital, Tianjin, China.
Bo Wu is a professor of General Thoracic Surgery, Lung Transplantation Group, Wuxi People's Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.Erlie Jiang is a professor of Blood Diseases hospital, Institute of Hematology, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.
Wen Li is a professor of Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Chen Meng is a professor of Qilu Children’s hospital of Shandong University, Jinan, Shandong, China.
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