Quantum Dots Applications in Biology

This third edition provides revised and expanded protocols of consolidated approaches as well as new trends in the field. Chapters guide readers through new approaches to optimize Quantum Dots’ (QD) properties, to evaluate their quantum yields, important features about preparative processes and characterizations of QDs, methods related to QDs for live cell applications, and the versatility of QDs in the bioanalytical and biosensing field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Part I: Tutorials

1. Multivariate Optimization: A Powerful Tool for the Systematic Control of Quantum Dots Properties

Isabelle Moraes Amorim Viegas, Giovannia Araujo de Lima Pereira, and Claudete Fernandes Pereira

2. Absolute and Relative Methods for Fluorescence Quantum Yield Evaluation of Quantum Dots

Renato E. de Araujo and Christian T. Dominguez

Part II: Preparative, Processes, and Characterizations

3. Preparation and Analysis of Quantum Dots – Applications of Capillary Electrophoresis

Karel Klepárník and Pavlína Modlitbová

4. Measuring the Hydrodynamic Radius of Colloidal Quantum Dots by Fluorescence Correlation Spectroscopy

Diogo B. Almeida and André A. de Thomaz

Alexander M. Saeboe, Joshua C. Kays, and Allison M. Dennis

6. Quantitative Analysis of Single Quantum Dot Trajectories

Oleg Kovtun, Lucas B. Thal, Travis C. Josephs, and Sandra J. Rosenthal

7. Phase Transfer and DNA Functionalization of Quantum Dots Using an Easy to Prepare, Low-Cost Zwitterionic Polymer

Margaret Chern, Chloe Grazon, and Allison M. Dennis

8. Dextran-Functionalized Quantum Dot-Immunoconjugates for Cellular Imaging

Kelly Rees, Melissa Massey, Michael V. Tran, and W. Russ Algar

Lucas B. Thal, Oleg Kovtun, and Sandra J. Rosenthal

10. Quantitative Chemical Delivery of Quantum Dots into the Cytosol of Cells

Joseph L. Watson, Alessandra Stangherlin, and Emmanuel Derivery

11. Stimulus-Sensitive Theranostic Delivery Systems Based on Microcapsules Encoded with Quantum Dots and Magnetic Nanoparticles

12. Resazurin-based Assay to Evaluate Cell Viability after Quantum Dot Interaction

Maria Isabela A. Pereira, Camila Aparecida P. Monteiro, Weslley Felix de Oliveira,

Beate S. Santos, Adriana Fontes, and Paulo E. Cabral Filho

Part IV: Bioanalysis

13. Multiplexed Detection of Cancer Serum Antigens with a Quantum Dot–Based Lab-on-Bead System

Tatiana Tsoy, Alexander Karaulov, Igor Nabiev, and Alyona Sukhanova

14. CdS Quantum Dots Modified Photoelectrochemical Biosensor for TATA-Binding Protein Probing

Yi-Fan Ruan, Xiao-Mei Shi, Hai-Yan Wang, Wei-Wei Zhao, Jing-Juan Xu, and Hong-Yuan Chen

15. Quantum Dots-based Lateral Flow Test Strip for Glutathione Detection

16. Comparative Advantages and Limitations of Quantum Dots in the Protein Array Applications

17. Development of Optical Sensors Based on Quantum Dots Using Molecularly Imprinted Polymers for Determination of Prilocaine

18. Detection of Tetanus Antibody Applying a Cu-Zn-In-S/ZnS Quantum Dots-based Lateral Flow Immunoassay

19. Quantum Dots for Assessment of Reactive Oxygen Species Accumulation during

Chemotherapy and Radiotherapy

Kimal Honour Djam, Bong Han Lee, Sindhuja Suresh, and Andrew Edet Ekpenyong