Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants

Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance. In Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, expert researchers provide detailed, step-by-step protocols for the establishment of in vitro cultures of key medicinal plants, their mass multiplication in a controlled environment, and step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers. In addition, many of these protocols will provide a basis for much needed efforts of in vitro germplasm conservation or cryopreservation of medicinal plant species at the brink of extinction as well as efforts to protect them from the adverse impact of rapid climatic changes. As a volume in the Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants is an ideal resource for scientists endeavoring to continue the research on this exciting natural branch of medicine.

Section A: Micropropagation and Chemical Analysis 1. Establishment of Adventitious Root Cultures of Echinacea purpurea for the Production of Caffeic Acid Derivatives Kee Yoeup Paek, Hosakatte Niranjana Murthy, and Eun Joo Hahn 2. In Vitro Propagation of Rauwolfia serpentina Using Liquid Medium, Assessment of Genetic Fidelity of Micropropagated Plants, and Simultaneous Quantization of Reserpine, Ajmaline and Ajmalicine M. K. Goel, S. Mehrotra, A. K. Kukreja, K. Shanker, and S.P.S. Khanuja 3. Medicinal Properties, in vitro Protocols and Secondary Metabolite Analyses of Scots Pine Hely Häggman, Anna Maria Pirttilä, Karoliina Niemi, Tytti Sarjala, and Riitta Julkunen-Tiitto 4. Saussurea medusa Cell Suspension Cultures for Flavonoid Production Chun-Zhao Liu and Praveen K. Saxena 5. Micropropagation of Crataeva nurvala Shashi B. Babbar, Neetika Walia, and Amandeep Kaur 6. Bilberry In vitro Protocols and Analyses of Phenolic Compounds Laura Jaakola, Kaisu Riihinen, Hely Häggman, and Anja Hohtola 7. In Vitro Propagation of Two Tuberous Medicinal Plants: Holostemma ada-kodien and Ipomoea mauritiana S. Pillai Geetha, AV. Raghu, Gerald Martin, Satheesh George, and Indira Balachandran 8. In Vitro Production of Gymnemic Acid from Gymnema sylvestre (Retz) R.Br. Ex Roemer & Schultes through Callus Culture Under Abiotic Stress Conditions Abdul Bakrudeen Ali Ahmed, Adhikarla Suryanarayana Rao, and Mandali Venkateswara Rao 9. Establishment of Plant Regeneration and Cryopreservation System from Zygotic Embryo-Derived Embryogenic Cell Suspension Cultures of Ranunculus kazusensis Suk Weon Kim and Myung Jin Oh 10. In vitro Culture and Secondary Metabolite Isolation in Bryophytes Aneta Sabovljevic, Marko Sabovljevic, and NebojsaJockovic 11. Micropropagation and In vitro Conservation of Vanilla (Vanilla planifolia Andrews.) Minoo Divakaran and K. Nirmal Babu 12. Protocol for In vitro Regeneration and Marker Glycoside Assessment in Swertia chirata Buch-Ham Sushma Kaul, K.A. Suri, P. Dutt, M. Sambyal, A. Ahuja, and M.K. Kaul 13. Protocols for Establishment of an In Vitro Collection of Medicinal Plants in the Genus Scutellaria Ian B. Cole, Faisal T. Farooq, and Susan J. Murch 14. Protocols for In Vitro Culture and Phytochemical Analysis of Phyllanthus Species (Euphorbiaceae) Elizabete Catapan, Fábio Netto Moreno, Márcio Luís Busi da Silva, Michel Fleith Otuki, Rivaldo Niero, Valdir Cechinel Filho, Rosendo Augusto Yunes, and Ana Maria Viana 15. In vitro Clonal Propagation of Asparagus racemosus, a High Value Medicinal Plant Sanjay Saxena and Nishritha Bopana 16. Micropropagation of Penthorum chinense through Axillary Bud Jun Yang and Zheng-song Peng Section B: Transgenic Approaches 17. Spontaneous Plant Regeneration and Production of Secondary Metabolites from Hairy Root Cultures of Centaurium erythraea Rafn Angelina Subotic, Sladana Jevremovic, Dragoljub Grubišic, Teodora Jankovic 18. Transgenic Hypericum perforatum G. Franklin, Margarida M. Oliveira, and Alberto C.P. Dias 19. Agrobacterium-Mediated Transformation of Ruta graveolens L. Karine Lièvre, Thi Lê Minh Tran, Sébastien Doerper, Alain Hehn, Paul Lacoste, Brigitte Thomasset, Frédéric Bourgaud, and Eric Gontier 20. Gene Expression Profiling in Taxus baccata L. Seedlings and Cell Cultures Katarína Brunáková and Ján Košuth 21. Catapol Production in Chinese Foxglove (Rehmannia glutinosa Libos.) Hairy Roots Transformed with