This book is an attempt to provide in a single source current state-of-the-art methodologies for protein sequence analysis. It is hoped that these various chapters are presented in such a way that both the newcomer and the established protein chemist will find useful information and directions to new techniques. This book offers a rich array of techniques and methods for sequencing proteins and peptides. It should meet the expectations of investigators in protein chemistry who wish to update their knowledge of sequencing techniques, and of those who wish to reacquaint themselves with the best available current technologies.
1. Purification of Proteins/Peptides for Structural Studies 2. Manual Methods for Protein/Peptide Sequence Analysis 3. Liquid Phase Sequence Analysis of Proteins/Peptides 4. Solid-Phase Sequence Analysis of Proteins/Peptides 5. Gas Phase Sequence Analysis of Proteins/Peptides 6. Homologous Edman-Type Reagents in Microsequence Analysis of Polypeptides 7. Chemical Methods of Amino Acid Sequence Analysis from Carboxyterminal End 8. Enzymatic Methods of Protein/Peptide Sequence from Carboxyterminal End 9. Protein/Peptide Sequence Analysis by Mass Spectroscopy 10. A Novel Approach to Amino Acid Sequencing 11. Analysis of Modified Amino Acids 12. Identification of Phenylthiohydantion Derivatives of Amino Acids
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