Polypeptide Materials Methods and Protocols

A Shortcut to the Synthesis of Peptide Thioesters Using an HF-Free Approach.- SnoopLigase-Mediated Peptide-Peptide Conjugation and Purification.- Peptide Nanoparticles for Gene Packaging and Intracellular Delivery.- Synthesis and Application of Peptide-siRNA Nanoparticles from Disulfide-Constrained Cyclic Amphipathic Peptides for the Functional Delivery of Therapeutic Oligonucleotides to the Lung.- FRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures.- Molecular Simulations Guidelines for Biological Nanomaterials: From Peptides to Membranes.- Functional Peptide Nanocapsules Self-Assembled from b-Annulus Peptides.- Electrostatic Self-Assembly of Protein Cage Arrays.- Design and Generation of Self-Assembling Peptide Virus-Like Particles with Intrinsic GPCR Inhibitory Activity.- Imaging and 3D Reconstruction of De Novo Peptide Capsids.- Monitoring the Assembly and Aggregation of Polypeptide Materials by Time-Resolved Emission Spectra.- Forming Low Molecular Weight Hydrogels by Electrochemical Methods.- In Situ Measurements of Polypeptide Samples by Dynamic Light Scattering: Membrane Proteins, a Case Study.- Measurement of Peptide Coating Thickness and Chemical Composition Using XPS.- Imaging the Effects of Peptide Materials on Phospholipid Membranes by Atomic Force Microscopy.- Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials.- Ultramicrotomy Analysis of Peptide-Treated Cells.