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klemencic marina (curatore); stael simon (curatore); huesgen pitter f. (curatore) - plant proteases and plant cell death

Plant Proteases and Plant Cell Death Methods and Protocols

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Lingua: Inglese


Pubblicazione: 05/2022
Edizione: 1st ed. 2022


This volume presents current methods to detect and measure the activity of proteolytic enzymes in organisms ranging from unicellular algae to flowering plants. Chapters detail in vitro production, characterization of plant proteases, tools for in vivo modifications, proteomic approaches for identification of substrates, inhibitors and interacting partners, and the function of plant proteases in plant programmed cell death. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.


Authoritative and cutting-edge, Plant Proteases: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. 


1          Expression and purification of the type I metacaspase from a cryptophyte Guillardia theta, GtMCA-I        

Vida Štrancar, Katarina Petra van Midden, Marina Klemencic, Christiane Funk


2          Expression and purification of the type II metacaspase from a unicellular green alga Chlamydomonas reinhardtii  

Igor Sabljic, Yong Zou, Marina Klemencic, Christiane Funk, Jerry Ståhlberg, Peter Bozhkov


3          Expression in Escherichia coli, refolding, and purification of plant aspartic proteases          
Pedro Castanheira, Carla Almeida, Daniela Dias-Pedroso, Isaura Simões


4          Production of functional plant legumain proteases using the Leishmania tarentolae expression system     

Elfriede Dall, Andreas Licht, Hans Brandstetter


5          Purification of His-tagged proteases from the apoplast of agroinfiltrated N. benthamiana          

Mariana Schuster, Judith K. Paulus, Jiorgos Kourelis, Renier A. L. van der Hoorn


6          Identification of cognate protease/substrate pairs by use of class-specific inhibitors      
Annick Stintzi, Nils Stührwohldt, Stefanie Royek,  Andreas Schaller


7          Peptide backbone modifications for the assessment of cleavage site relevance in precursors of signaling peptides     
Xu Wang, Jens Pfannstiel, Annick Stintzi and Andreas Schaller


8          Detection of apoplastic protease inhibitors using convolution activity based proteome profiling      

Andrea Passarge, Gunther Doehlemann, Johana C. Misas Villamil


9          Cysteine reactivity profiling to unveil redox regulation in phytopathogens

Kyoko Morimoto, Monika Stegmann, Farnusch Kaschani, Shabaz Mohammed, Renier A. L. van der Hoorn


10        Determination of caspase-like activities in roots by the use of fluorogenic substrates            

Katarina Šoln, Marina Klemencic


11        Detection of damage-activated metacaspase activity by Western blot in plants

Simon Stael, Luke P. Miller, Álvaro D. Fernández-Fernández, Frank Van Breusegem


12        Sensitive plant N-terminome profiling with HUNTER        
Fatih Demir, Andreas Perrar, Melissa Mantz, Pitter F. Huesgen


13        Profiling sequence specificity of proteolytic activities using proteome-derived peptide libraries   

Fatih Demir, Maithreyan Kuppusamy, Andreas Perrar, Pitter F. Huesgen


14        Monitoring Cell Death via Ion leakage and PAM Fluorometry
Nick Dunken, Lisa Mahdi, Rainer E. Häusler, Alga Zuccaro


15        Measuring and perturbing ferroptosis in plants         

Ayelen M. Distéfano, Fernanda Marchetti, Eduardo Zabaleta, Gabriela C. Pagnussat


16        Detection and quantification of the hypersensitive response cell death in Arabidopsis thaliana
Jose Salguero-Linares, Saul Lema-Asqui, Marta Salas-Gómez, Andrea Froilán-Soares, Núria S. Coll


17        Monitoring of ATG4 protease activity during autophagy in the model microalga Chlamydomonas reinhardtii          

José Luis Crespo, Maria Esther Pérez-Pérez


18        Oxidative stress in roots – detection of lipid peroxidation and total antioxidative capacity    

Katarina Šoln, Jasna Dolenc Koce


19        Imaging of potassium and calcium distribution in plant tissues and cells to monitor stress response and programmed cell death     
Katarina Vogel-Mikuš, Paula Pongrac


20        Monitoring Pseudomonas syringae growth in agroinfiltrated leaves: the ‘agromonas’ assay

Pierre Buscaill, Renier A. L. van der Hoorn


21        Analysis of virus spread around the cell death zone at spatiotemporal resolution using confocal microscopy          
Tjaša Lukan, Anna Coll, Špela Baebler, Kristina Gruden


22        A user guide to validation, annotation and evaluation of N-terminome datasets with MANTI      
Fatih Demir, Pitter F. Huesgen


23        Exploring posttranslational modifications with the Plant PTM Viewer
Patrick Willems


Altre Informazioni



Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 254 x 178 mm Ø 787 gr
Formato: Copertina rigida
Illustration Notes:XI, 299 p. 58 illus., 53 illus. in color. With online files/update.
Pagine Arabe: 299
Pagine Romane: xi

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