Plant Photomorphogenesis Methods and Protocols

This book provides detailed protocols for research in plant photomorphogenesis. The collection includes a broad range of topics including assays for shade avoidance responses, assays for light-dependent protein-protein interactions, photobody detection with immunofluorescence and the super-resolution imaging method, protein complex isolation from plants, detection of homodimer and monomer of photoreceptor UVR8 with immunoblotting analysis, assays for seedling greening, procedures for studying skotomorphogenesis, phenotypic study of photomorphogenesis at the seedling stage, expression of Cryptochrome in insect cells, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

1. Setting up an Arabidopsis LED Culture Module that Simulates Plant Neighbor Proximity

            Carlos D. Crocco

 

2. Photobody Detection Using Immunofluorescence and Super-Resolution Imaging in Arabidopsis

            Giorgio Perrella, Anna Zioutopoulou, Andrew Hamilton, and Eirini Kaiserli

 

3. Analysis of Shade-Induced Hypocotyl Elongation in Arabidopsis

            Yetkin Çaka Ince and Vinicius Costa Galvão

 

4. Isolation of UVR8 Protein Complexes

            Yan Liu and Xi Huang

 

5. Phenotypic Study of Photo-Morphogenesis in Arabidopsis Seedlings

            Chuanwei Yang, Famin Xie, and Lin Li

 

6. Experimental Procedures for Studying Skotomorphogenesis in Arabidopsis thaliana

            Huanhuan Jin, Hong Li, and Ziqiang Zhu

 

7. Global Identification for Targets of Circadian Transcription Factors in Arabidopsis and Rice Using Chromatin Immunoprecipitation Followed by Sequencing (ChIP-seq)

            Shuxuan Xu, Jing Huang, Jian Jin, and Wei Huang

 

8. Co-Immunoprecipitation Assays to Detect In Vivo Association of Phytochromes with Their Interacting Partners

            Pengyu Song, Shaoman Zhang, and Jigang Li

 

9. Detection of UVR8 Homodimers and Monomers by Immunoblotting Analysis in Tomato

            Guoqian Yang, Xiaorui Liu, and Li Lin

 

10. Characterization of Seedling Greening Process in Plant Photomorphogenesis

            Wanqing Wang, Yuhong Li, and Rongcheng Lin

 

11. Protoplast System for Studying Blue-Light-Dependent Formation of Cryptochrome Photobody

            Xiangguang Lyu, Hongyu Li, and Bin Liu

 

12. Uncover the Nuclear Proteomic Landscape with Enriched Nuclei Followed by Label-Free Quantitative Mass Spectrometry

            Yan Wang, Zhuang Lu, and Lei Wang

 

13. Strategies to Study Dark Growth Deficient or Slower Mutants in Chlamydomonas reinhardtii

            Huanling Yang, Fei Han, Yue Wang, Wenqiang Yang, and Wenfeng Tu

 

14. Co-Immunoprecipitation Assay for Blue Light-Dependent Protein Interactions in Plants

            Jingyi Zhang and Shengbo He

 

15. Detecting Blue Light-Dependent Protein-Protein Interactions by LexA-Based Yeast Two-Hybrid Assay

            Xiaolong Hao and Ling Li

 

16. Express Arabidopsis Cryptochrome in Sf9 Insect Cells Using the Baculovirus Expression System

            Xu Li, Yawen Liu, and Hongtao Liu

 

17. Semi-In-Vivo Pull Down Assay for Blue Light-Dependent Protein Interactions

            Xu Li, Yawen Liu, and Hongtao Liu

 

18. Tobacco System for Studying Protein Colocalization and Interactions

            Jingyi Zhang and Shengbo He