Plant Pathology Techniques and Protocols

Plant diseases can have an enormous impact on our lives. In a world where total crop failure can quickly lead to human misery and starvation, accurate diagnostics play a key role in keeping plants free from pathogens. In Plant Pathology: Techniques and Protocols, expert researchers provide methods which are vital to the diagnosis of plant diseases across the globe, addressing all three categories of plant pathology techniques: traditional, serological, and nucleic acid. Chapters examine recent and developing issues with crop identity and authenticity, allowing workers to genotype samples from two major food groups. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Authoritative and reader-friendly, Plant Pathology: Techniques and Protocols is an incredible guide which will soon prove to be indispensable, both to novices and expert researchers alike.

Table of Contents- Plant Pathogens: Techniques and Protocols 1. Bioassay for Diagnosis of Plant Viruses Isla A. Browning 2. Development of Enzyme Linked, Tissue Blot and Dot Blot Immunoassays for Plant Virus Detection Hei-ti Hsu 3. Immunisation strategies for Antibody Production Robert Burns 4. Preparation of Immunogens and Production of Antibodies Hei-ti Hsu, T. C. Chen, C. A. Chang, S. D. Yeh 5. Enzyme-Linked Immunosorbent Assay (ELISA) detection of Ralstonia solanacearum In Potatoes: The South African Experience Dirk U. Bellstedt 6. Production of Monoclonal Antibodies to Plant Pathogens Christopher R. Thornton 7. Enzyme-linked Immunosorbent Assay (ELISA) for the detection and identification of plant pathogenic bacteria (in particular for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus) Blanka Kokoskova and J. D. Janse 8. Indirect Immunofluorescence Microscopy (IIF) for the Detection and Identification of Plant Pathogenic Bacteria Jaap D. Janse and B. Kokoskova 9. Detection of Four Major Bacterial Potato Pathogens Ellen M. Kerr, G. Cahill and K. Fraser 10. Erwinia amylovora: Modern Methods for Detection and Differentiation Antonet M. Svircev, W. S. Kim, S. M. Lehman and A. J. Castle 11. The use of Fluorescent in situ Hybridisation in Plant Fungal Identification and Genotyping Mohamed Hijri 12. Use of Molecular Methods for the Detection of Fungal Spores Elaine Ward 13. Identification of Phytophthora fragariae var. rubi by PCR Alexandra Schlenzig 14. Detection of Double-stranded RNA Elements in the Plant Pathogenic Fungus Rhizoctonia solani Nikki D. Charlton, S. M. Tavantzis and M. A. Cubeta 15. Immunocapture-PCR for Plant Virus Detection Vincent Mulholland 16. Multiplex Polymerase Chain Reaction (PCR) and Real-time Multiplex PCR for the Simultaneous Detection of Plant Viruses Vicente Pallas, J. Sanchez-Navarro, A. Varga, F. Aparicio and D. James 17. Fluorescent-based Techniques for Viral Detection, Quantification and Characterisation Mathieu Rolland, A. Delauney and E. Jacquot 18. Analysis of Population Structures of Viral Isolates using Single Strand Conformation Polymorphism (SSCP) Method Agnes Delauney, M. Rolland and E. Jacquot 19. Direct Detection of Plant Viruses in Potato Tubers using Real-time PCR Neil Boonham, L. Laurenson, R. Weekes, and R. Mumford 20. An Accelerated Soil Bait Assay for the Detection of Potato Mop Top Virus (PMTV) in Agricultural Soil. Triona Davey 21. Detection of Phytoplasmas of Temperate Fruit Trees Margit Laimer 22. PCR Detection of Potato Cyst Nematode Alex Reid 23. Potato Cultivar Genome Analysis Alex Reid, L. Hof, D. Esselink and B Vosman 24. Barley Variety Identification using SSRs Cathy Southworth