Part I: In Vitro and In Vivo Models to Study Photodynamic Therapy
1. Application of Monolayer Cell Cultures for Investigating Basic Mechanisms of Photodynamic Therapy
Mans Broekgaarden
2. The Negative Impact of Cancer Cell Nitric Oxide on Photodynamic Therapy
Jonathan M. Fahey and Albert W. Girotti
3. Microtumor Models as a Preclinical Investigational Platform for Photodynamic Therapy
Mans Broekgaarden and Jean-Luc Coll
4. A Microfluidic Model to Evaluate Photodynamic Therapy Treatment Response in 3D Tumors
Shubhankar Nath, Michael Pigula, Tayyaba Hasan, and Imran Rizvi
5. Analysis of Treatment Effects on Structurally Complex Microtumor Cultures Using a Comprehensive Image Analysis Procedure
Anne-Laure Bulin, Mans Broekgaarden, and Tayyaba Hasan
6. High-Throughput Examination of Therapy-Induced Alterations in Redox Metabolism in Spheroid and Microtumor Models
Mans Broekgaarden, Anne-Laure Bulin, and Tayyaba Hasan
7. Spatiotemporal Tracking of Different Cell Populations in Cancer Organoid Models for Investigations on Photodynamic Therapy
Anne-Laure Bulin and Tayyaba Hasan
8. Generating Large Numbers of Pancreatic Microtumors on Alginate-Gelatin Hydrogels for Quantitative Imaging of Tumor Growth and Photodynamic Therapy Optimization
Nazareth Milagros Carigga Gutierrez, Tristan Le Clainche, Jean-Luc Coll, Lucie Sancey, and Mans Broekgaarden
9. The Chicken Embryo Chorioallantoic Membrane as an In Vivo Model for Photodynamic Therapy
Jaroslava Joniová and Georges Wagnières
10. Subcutaneous Xenograft Models for Studying PDT In Vivo
Girgis Obaid and Tayyaba Hasan
11. In Vivo Models for Studying Interstitial Photodynamic Therapy of Locally Advanced Cancer
Gal Shafirstein, Emily Oakley, Sasheen Hamilton, Michael Habitzruther, Sarah Chamberlain, Sandra Sexton, Leslie Curtin, and David A. Bellnier
12. Orthotopic Models of Pancreatic Cancer to Study PDT
Girgis Obaid, Zhiming Mai, and Tayyaba Hasan
13. An Orthotopic Murine Model of Peritoneal Carcinomatosis of Ovarian Origin for Intraoperative PDT
Thierry Michy, Claire Bernard, Jean Luc Coll, and Véronique Josserand
14. Photodynamic Treatments for Disseminated Cancer Metastases Using Fiber-Optic Technologies
Eric M. Kercher and Bryan Q. Spring
15. Stereotaxic Implantation of F98 Cells in Fischer Rats: A Syngeneic Model to Investigate Photodynamic Therapy Response in Glioma
Anne-Laure Bulin, Jean-François Adam, and Hélène Elleaume
Part II: Inorganic Photosenstizers and Light-Activatable Anti-Cancer Prodrugs
16. New Generation of Photosensitizers Based on Inorganic Nanomaterials
Xiaomin Liu and Hong Zhang
17. Cytotoxicity of Metal-Based Photoactivated Chemotherapy (PACT) Compounds
Vadde Ramu, Austin B. Auyeung, and Sylvestre Bonnet
Part III: Third and Fourth-Generation Photosensitizers
18. Photosensitized Oxidation of Intracellular Targets: Understanding the Mechanisms to Improve the Efficiency of Photodynamic Therapy
Thiago Teixeira Tasso and Maurício da Silva Baptista
19. Inhibition of the HIF-1 Survival Pathway as a Strategy to Augment Photodynamic Therapy Efficacy
Mark J. de Keijzer, Daniel J. de Klerk, Lianne R. de Haan, Robert T. van Kooten, Leonardo P. Franchi, Lionel M. Dias, Tony G. Kleijn, Diederick J. van Doorn, and Michal Heger on behalf of the International Photodynamic Therapy Study Group
20. Strategies for Improving Photodynamic Therapy through Pharmacological Modulation of the Immediate Early Stress Response
Daniel J. de Klerk, Mark J. de Keijzer, Lionel M. Dias, Jordi Heemskerk, Lianne R. de Haan, Tony G. Kleijn, Leonardo P. Franchi, and Michal Heger on behalf of the International Photodynamic Therapy Study Group
21. Nanobody-Targeted Photodynamic Therapy: Nanobody Production and Purification
Vida Mashayekhi, Erik Schooten, Paul M.P. van Bergen en Henegouwen, Marta M. Kijanka, and Sabrina Oliveira
22. Conjugation of IRDye Photosensitizers or Fluorophores to Nanobodies
Vida Mashayekhi and Sabrina Oliveira
23. In Vitro Assessment of Binding Affinity, Selectivity, Uptake, Intracellular Degradation, and Toxicity of Nanobody-Photosensitizer Conjugates
Irati Beltrán Hernández, Timo W.M. De Groof, Raimond Heukers, and Sabrina Oliveira
24. Assessment of the In Vivo Response to Nanobody-Targeted PDT through Intravital Microscopy
Henriette S. de Bruijn, Ann L.B. Seynhaeve, Timo L.M. ten Hagen, Sabrina Oliveira, and Dominic J. Robinson
25. Orthotopic Breast Cancer Model to Investigate the Therapeutic Efficacy of Nanobody-Targeted Photodynamic Therapy
Marion M. Deken, Shadhvi S. Bhairosingh, Alexander L. Vahrmeijer, and Sabrina Oliveira
26. Investigation of the Therapeutic Potential of Nanobody-Targeted Photodynamic Therapy in an Orthotopic Head and Neck Cancer Model
Pieter B.A.A. van Driel, Stijn Keereweer, Clemens W.G.M. Lowik, and Sabrina Oliveira
Part IV: Photodynamic Therapy-Induced Immune Signaling
27. Evaluation of the Antitumor Immune Response Following Photofrin-Based PDT in Combination with the Epigenetic Agent 5-Aza-2’-Deoxycytidine
Malgorzata Wachowska, Angelika Muchowicz, and Jakub Golab
28. Controlling Immunoregulatory Cell Activity for Effective Photodyna
