Oxytocin - Werry Eryn L. (Curatore); Reekie Tristan A. (Curatore); Kassiou Michael (Curatore) | Libro Humana 09/2021 - HOEPLI.it

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werry eryn l. (curatore); reekie tristan a. (curatore); kassiou michael (curatore) - oxytocin

Oxytocin Methods and Protocols

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Lingua: Inglese


Pubblicazione: 09/2021
Edizione: 1st ed. 2022


This detailed book explores techniques for further elucidating the peripheral and central roles of oxytocin as well as techniques key to oxytocin receptor-related drug discovery. The first set of chapters explore this neuropeptide’s peripheral and central effects, such as regulation of myometrial contraction, induction of cardioprotective effects, and the facilitation of pro-social behaviors. The book then continues by delving into a comprehensive pharmacological characterization of oxytocin receptor ligands and ligands of other key receptors such as the vasopressin receptor family. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Oxytocin: Methods and Protocols is an ideal guide for researchers seeking to further our knowledge of the varied and power effects of oxytocin within the central nervous system.


1. The Importance of Experimental Investigation of the Peripheral Oxytocin System

            Stephen J. Assinder


2. Human Myometrial Contractility Assays

            Sarah Arrowsmith


3. The Effect of Oxytocin on Intracellular Ca2+ Release in Cardiac Cells

            Anatoliy Shmygol, Sanaa H.S.H. Alameri, Khadeijah Y.K.M. Almemari, Naema M.A.J. Alsuwaidi, and Ifrah I. Ali


4. The Importance of Experimental Investigation of the CNS Oxytocin System

            Kelsie A. Boulton and Adam J. Guastella


5. The Role of Oxytocin in Social Circuits and Social Behavior in Dementia

            Olivier Piguet, Rebekah M. Ahmed, and Fiona Kumfor


6. Evaluating Methylation of the Oxytocin Receptor Gene and the Oxytocin Intergenic Region

            Leonora King, Stephanie Robins, Gary Chen, Gustavo Turecki, and Phyllis Zelkowitz


7. Using Receptor Autoradiography to Visualize and Quantify Oxytocin and Vasopressin 1a Receptors in the Human and Nonhuman Primate Brain

            Sara M. Freeman


8. Linking Central Gene Expression Patterns and Mental States Using Transcriptomics and Large-Scale Meta-Analysis of fMRI Data: A Tutorial and Example Using the Oxytocin Signaling Pathway

            Jaroslav Rokicki, Daniel S. Quintana, and Lars T. Westlye


9. CRISPR/Cas9-Mediated Genetic Engineering to Generate a Disease Model Prairie Vole, Based on the Species-Optimized Assisted Reproductive Technology

            Kengo Horie and Katsuhiko Nishimori


10. The Current Status of Drug Discovery for the Oxytocin Receptor

            Philippe E. Nashar, Aidan Whitfield, Jiri Mikusek, and Tristan A. Reekie


11. Fmoc Solid Phase Peptide Synthesis of Oxytocin and Analogues

            Thomas Kremsmayr and Markus Muttenthaler


12. Employing the Operational Model to Measure System-Independent OTR Efficacy

            Kiyan Afzali and Mark Connor


13. Use of BRET to Measure ß-Arrestin Recruitment at Oxytocin and Vasopressin Receptors

            Edin Muratspahic, Jasmin Gattringer, and Christian W. Gruber


14. Measuring Affinity of Ligands to the Oxytocin Receptor Using Radioligand Binding

            Eryn L. Werry and Michael Kassiou


15. Use of Co-Cultures to Measure the Blood-Brain Barrier Permeability of Oxytocin

            Yasuhiko Yamamoto, Ai Harashima, Sei-ichi Munesue, and Haruhiro Higashida


16. Intracerebral Delivery of an OTR Antagonist and Subsequent Behavioral Testing in Prairie Voles

            Yu Hirota, Chisato Otsuki, Erika Kamura, and Shinichi Mitsui

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Altre Informazioni



Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 254 x 178 mm Ø 736 gr
Formato: Copertina rigida
Illustration Notes:9 Illustrations, black and white
Pagine Arabe: 272
Pagine Romane: xi

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