Osteoporosis and Osteoarthritis

In Osteoporosis and Osteoarthritis expert investigators detail modern tools to enhance discovery in bone and cartilage biology and promote translational research in osteoporosis and osteoarthritis. New chapters describe methods to generate induced pluripotent stem cells, perform high-throughput chromatin analysis, assess loads on bone cells in vivo and in vitro, use state-of-the art imaging techniques to measure articular cartilage thickness and biochemical properties, and establish animal models of osteoarthritis and bone healing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical,  Osteoporosis and Osteoarthritis shares successful techniques that can be applied to the study of either disease.

 Part I: Cell Biology

 1.  Isolation Of Osteocytes From Mature and Aged Murine Bone

            Amber Rath Stern and Lynda F. Bonewald

2.  Primary Murine Growth Plate and Articular Chondrocyte Isolation and Culture

            Jennifer H. Jonason, Donna Hoak, and Regis J. O’Keefe

3.  Isolating Endosteal Mesenchymal Progenitors From Rodent Long Bones

            Ji Zhu, Valerie A. Siclari, and Ling Qin

4.  Engineering Cartilage Tissue By Pellet Co-Culture Of Chondrocytes And Mesenchymal Stromal Cells

            Ling Wu, Janine N. Post, and Marcel Karperien

5.  Generation Of Induced Pluripotent Stem Cells

            David R. Deyle

Part II: Molecular Biology

 6.  Profiling Histone Modifications By Chromatin Immunoprecipitation Coupled To Deep Sequencing in Skeletal Cells

            Mark B. Meyer, Nancy A. Benkusky, and J. Wesley Pike

7.    Identification Of Micrornas In Human Plasma

Bram C. Van Der Eerden, Rodrigo D. Alves, Christel E. Kockx, Zeliha Ozgur, Marijke Schreuders-Koedam, Jeroen Van De Peppel, Wilfred F. Van Ijcken, and Johannes P. Van Leeuwen

8.    Immunohistochemistry Of Skeletal Tissues

        Crystal Idleburg, Elizabeth N. Delassus, And Deborah V. Novack

Part III: Biomechanics and Imaging

 9.    In Vivo Axial Loading Of The Mouse Tibia

Katherine M. Melville, Alexander G. Robling, and Marjolein C. H. Van Der Meulen

10.  Four Point Bending Protocols To Study The Effects Of Dynamic Strain In Osteoblastic Cells In Vitro

        Gabriel L. Galea and Joanna S. Price

11.  Epic-µct Imaging Of Articular Cartilage

Angela S.P. Lin, Giuliana E. Salazar-Noratto, and Robert E. Guldberg

Part IV: In Vivo Models Of Disease and Repair

 12.  Mouse Models Of Osteoarthritis: Surgical Model Of Post-Traumatic Osteoarthritis Induced By Destabilization Of The Medial Meniscus

Cecilia L. Dragomir, Jun Chang, Elisabeth B. Wondimu, Jonathan Coico, Darren A. Plumb, Miguel Otero, Kirsty L. Culley, and Mary B. Goldring

13.   Assessment Of Knee Joint Pain In Experimental Rodent Models Of Osteoarthritis

            Margaret J. Piel, Jeffrey S. Kroin, and Hee-Jeong Im

14.  Induction Of Fully Stabilized Cortical Bone Defects To Study Intramembranous Bone Regeneration

Meghan E. Mcgee-Lawrence and David F. Razidlo

15.  Surgical Procedures And Experimental Outcomes Of Closed Fractures In Rodent Models Hicham Drissi and David N. Paglia