Non-Natural Nucleic Acids Methods and Protocols

Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs.- Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites, 8-Oxo-7,8-Dihydro-2’-Deoxyguanosine and 2’-Deoxycytidine.- Synthesis of a Fluorescent Cytidine TNA Triphosphate Analogue.- Synthesis of Base-Modified dNTPs through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA.- 2'-C,4'-C-Ethyleneoxy-Bridged 2'-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability.- Synthesis Protocols for Simple Uncharged Glycol Carbamate Nucleic Acids.- Synthesis of Nucleobase-Functionalized Morpholino Monomers.- Synthesis and Application of ^LK?T Peptide Nucleic Acids.- Aminoglycoside Functionalization as a Tool for Targeting Nucleic Acids.- Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically-Stable Covalent Interstrand Cross-Link between a Guanine Residue and an Abasic Site.- Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-Modified Nucleic Acids.- Labeling Peptide Nucleic Acids with Indium-111.- Site-Specific Labeling of DNA via PCR with an Expanded Genetic Alphabet.- Flexible Nucleic Acids (FNAs) As Informational Molecules:  Enzymatic Polymerization of fNTPs on DNA Templates and Nonenzymatic Oligomerization of RNA on FNA Templates.- Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis.- FRET Assay for Ligands Targeting the Bacterial A-Site RNA.- The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons.- Oligonucleotide Primers with G^8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA.- Determining Steady-State Kinetics of DNA Polymerase Nucleotide Incorporation.