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boulton alan a. (curatore); baker glen b. (curatore); hefti franz (curatore) - neurotrophic factors

Neurotrophic Factors

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Dettagli

Genere:Libro
Lingua: Inglese
Editore:

Humana

Pubblicazione: 08/2013
Edizione: Softcover reprint of the original 1st ed. 1993





Trama

The field of neurotrophic factors has witnessed exp- sive growth in the past decade. As is usual in scientific in- vation, this progress has been closely associated with methodological advances. The introduction of molecular b- logical techniques into the neurotrophic factor field led to the discovery of new families of neurotrophic growth f- tors and their receptors. Production of growth factors by recombinant technology played a crucial part. The example of nerve growth factor, the paradigmatic neurotrophic factor, illustrates this point. A decade ago investigators were forced to purify small quantities of this protein from murine salivary glands, but much larger qu- tities of recombinant nerve growth factor are now available for experimentation as well as clinical development. A decade ago there was a controversy about the existence of nerve growth factor in the brain and the immunoassays used for its measurement, but current publications report the precise localization of gene expression for nerve growth factor and its receptor in the brain. Neurotrophic Factors aims at presenting the techniques that have been crucial to the realization of these rapid advances and thus have helped propel the neurotrophic factors field to its current status of high visibility. These techniques range from molecular biological methods used for cloning and production, to cell culture methods for assessing biological activities, to animal models of nervous system injury (nec- sary for the development of therapeutic agents from neurotrophic factors).




Sommario

Fischer/Schubert: Purification and Microsequencing of Neurotrophic Proteins. Nikolics: Identification of Neurotrophic Factors by Molecular Biological Techniques. Persson/Ernfors: Visualization and Quantitation of Neurotrophin mRNAs. Heinrich/Martin: Molecular Analysis of Neurotrophin Genes. Burton: Production of Recombinant Neurotrophic Factors. Ray/Kawaja/Gage: Production of Genetically Engineered Cells Releasing Neurotrophic Factors. Richardson/Verge/Riopelle: Characterization and Localization of Receptors for Neurotrophic Factors. Huber/Chao: Transgenic Expression of Neurotrophic Factors and Their Receptors. Koliatsos/Price: Retrograde Axonal Transport. Hefti et al.: Experimental Systems to Study Neurotrophic Factor Effects on Rat Brain Cells. Koliatsos/Price: Nonhuman Primate Models in Trophic Factor Research. Varon/Hagg: Models to Evaluate Effects of Neurotrophic Factors on Axonal Regeneration. Index.










Altre Informazioni

ISBN:

9781489939876

Condizione: Nuovo
Collana: Neuromethods
Dimensioni: 229 x 152 mm Ø 646 gr
Formato: Brossura
Illustration Notes:XVIII, 422 p.
Pagine Arabe: 422
Pagine Romane: xviii


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