Natural Killer (NK) Cells Methods and Protocols

Isolation of Innate Lymphoid Cells from Murine Intestinal Lamina Propria.- Purification of Primary Decidual Natural Killer Cells for Functional Analysis.- In Vitro Development of Mouse and Human NK Cells from Hematopoietic Progenitor Cells.- Induction of Human Natural Killer Cells under Defined Conditions by Seamless Transition from Maintenance Culture of Pluripotent Stem Cells.- Development of Humanized Mouse Models for Studying Human NK Cells in Health and Disease.- Antibody-Oligonucleotide Conjugation Using a SPAAC Copper-Free Method Compatible with 10x Genomics’ Single-Cell RNA-Seq.- Methods to Analyze the Developmental Trajectory of Human Primary NK Cells Using Monocle and SCENIC Analyses.- Methods for Isolating and Defining Single-Cell Transcriptomes of Tissue-Resident Human NK Cells.- Retrogenic Color-Barcoding for Fate Mapping of Single Innate Lymphocytes.- Quantifying Human Natural Killer Cell Migration by Imaging and Image Analysis.- Tracking of NK Cells by Positron Emission Tomography Using ⁸⁹Zr-Oxine Ex Vivo Cell Labeling.- Assessing Changes in Human Natural Killer Cell Metabolism Using the Seahorse Extracellular Flux Analyzer.- Determining Activation Status of Natural Killer Cells Following Stimulation via Cytokines and Surface Receptors.- Method to Study Adaptive NK Cells Following MCMV Infections.- Assessing the Response of Human NK Cell Subsets to Infection by Clinically-Isolated Virus Strains.- NK Cell Isolation and Cytotoxicity by Radioactive Chromium Release Assay and DELFIA -EuTDA Cytotoxicity Assay.- Organoid Co-Culture Methods to Capture Cancer Cell -Natural Killer Cell Interactions.- In Vitro Visualization of Cell-to-Cell Interactions between Natural Killer Cells and Sensory Neurons.- CRISPR Screen to Identify Factors that Render Tumor Cells Sensitive or Resistant to Killing by NK Cells.- Practical Considerations and Workflow in Utilizing KIR Genotyping in Transplantation Medicine.- Gene Transductionof Natural Killer Cells for Clinical Application.