Monoamine Oxidase Methods and Protocols

This detailed book examines the main methods to study mammalian monoamine oxidases (MAOs), ranging from cell biology to computational chemistry. Beginning with techniques on how to obtain pure samples of MAO A and MAO B, the volume continues by covering assays and techniques used to measure MAO enzymatic activity and perform inhibition studies, methods to address cellular localization and function of MAOs, either in cell lines or in animal models, as well as computational methods applied to rational drug design approaches that are used to develop new MAO inhibitors. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

1. Purification of MAO A and of MAO B from Mammalian Tissue Sources

            Dale E. Edmondson

 

2. Purification of Recombinant Eukaryotic MAO A and MAO B Utilizing the Pichia pastoris Expression System

            Dale E. Edmondson

 

3. The Peroxidase-Coupled Assay to Measure MAO Enzymatic Activity

            Joana Reis and Claudia Binda

 

4. Measurement of MAO Enzymatic Activity by Spectrophotometric Direct Assays

            Joana Reis and Claudia Binda

 

5. Radiochemical Assay of Monoamine Oxidase Activity

            Andrew Holt

 

6. MAO Visible Spectroscopy for Ligand Interactions, Redox Chemistry, and Kinetics of Irreversible Inhibition

            Rona R. Ramsay

 

7. Conventional Receptor Radioligand Binding Techniques Applied to the Study of Monoamine Oxidase

            Andrew Holt

 

8. Assay of MAO Inhibition by Chromatographic Techniques (HPLC/HPLC-MS)

            Tomás Herraiz

 

9. Crystallization of Human Monoamine Oxidase B

            Claudia Binda, Dale E. Edmondson, and Andrea Mattevi

 

10. Detecting Monoamine Oxidase A and B Proteins: A Western Blotting Protocol and Some Practical Considerations

            Jennifer N.K. Nyarko, Ryan M. Heistad, Paul R. Pennington, and Darrell D. Mousseau

 

11. An (Immune)Fluorescence Protocol for Monitoring Monoamine Oxidase A/B Protein Distribution within the Cell

            Tyler J. Wenzel, Jennifer N.K. Nyarko, Ryan M. Heistad, Paul R. Pennington, Chris P. Phenix, and Darrell D. Mousseau

 

12. Expression and Function of MAO A in Cardiac Cells by Means of Adenovirus-Mediated Gene-Transfer

            Yohan Santin, Angelo Parini, and Jeanne Mialet-Perez

 

13. In Vitro and In Vivo Assays Characterizing MAO A Function in Cancers

            Boyang Jason Wu and Jean C. Shih

 

14. In Vivo Study of Monoamine Oxidases Using Multisite Intracerebral Microdialysis

            Philippe De Deurwaerdère, Nouhad Samb, Hasna El Boukhari, Rémi Corne, Abdeslam Chagraoui, and Giuseppe Di Giovanni

 

15. Informed Use of 3D-QSAR for the Rational Design of Coumarin Derivatives as Potent and Selective MAO B Inhibitors

            Nicola Gambacorta, Marco Catto, Leonardo Pisani, Angelo Carotti, and Orazio Nicolotti

 

16. Hansch-Type QSAR Models for the Rational Design of MAO Inhibitors: Basic Principles and Methodology

            Leonardo Pisani, Modesto de Candia, Mariagrazia Rullo, and Cosimo D. Altomare

 

17. Computational Chemistry and Molecular Modeling of Reversible MAO Inhibitors

            Kemal Yelekçi and Safiye Sag Erdem