Molecular Detection of Human Parasitic Pathogens

Traditionally, laboratory identification of parasites has relied upon various phenotypic procedures that detect their morphological, biological, and immunological features. Because these procedures tend to be time-consuming and technically demanding, molecular methods based on nucleic acid amplification technologies have been increasingly utilized for rapid, sensitive, and specific characterization of parasites. The large number of original and modified molecular protocols that have been developed over the years creates a dilemma for those attempting to adopt the most appropriate protocol for streamlined identification and detection of human pathogenic organisms of interest. Part of a four-volume collection, Molecular Detection of Human Parasitic Pathogens provides a reliable and comprehensive resource on the molecular detection and identification of major human parasitic pathogens. This volume contains expert contributions from international scientists involved in human parasitic pathogen research and diagnosis. Following a similar format throughout, each chapter includes: A brief review on the classification, biology, epidemiology, clinical features, and diagnosis of an important pathogenic parasitic genus/group An outline of clinical sample collection and preparation procedures and a selection of representative stepwise molecular protocols A discussion on further research needs relating to improved diagnoses of major human parasitic pathogens This versatile reference on molecular detection and identification of major human parasitic pathogens is an indispensable tool for upcoming and experienced medical, veterinary, and industrial laboratory scientists engaged in parasite characterization. It is also suitable as a textbook for undergraduate and graduate students majoring in parasitology.

Introductory RemarksDongyou LiuSection I SarcomastigophoraAcanthamoebaHélene Yera, Muriel Cornet, and Jean-Louis BourgesBalamuthiaGovinda S. Visvesvara and Yvonne QvarnstromBlastocystisHisao YoshikawaDientamoebaDamien StarkEntamoebaDamien Stark and John EllisGiardiaYaoyu Feng and Lihua XiaoLeishmaniaClotilde Marín, Silvia S. Longoni, and Manuel Sánchez-MorenoNaegleriaMineko Shibayama, Jesús Serrano-Luna, Isaac Cervantes-Sandoval, and Víctor TsutsumiPentatrichomonasTania CrucittiTrichomonasDongyou Liu, Xiangang Kong, and Changyou XiaTrypanosomaEmily R. AdamsSection II Ciliophora and ApicomplexBabesiaDongyou Liu and Xuedong LuBalantidiumDongyou LiuCryptosporidiumUna M. Ryan and Rongchang YangCyclosporaDongyou LiuIsosporaChaturong Putaporntip and Somchai JongwutiwesPlasmodiumDongyou Liu and Yi-Wei TangSarcocystisL. Vangeel, K. Houf, P. Geldhof, J. Vercruysse, R. Ducatelle, and K. ChiersToxoplasmaKjetil Asbakk and Kristin Wear PrestrudSection III Platyhelminthes: CestodaDiphyllobothriumBarbara Wicht, Raffaele Peduzzi, Hélene Yera, and Jean Dupouy-Camet DipylidiumDongyou LiuEchinococcus and EchinococcosisAkira Ito, Tetsuya Yanagida, Yasuhito Sako, Minoru Nakao, Kazuhiro Nakaya, Jenny Knapp, and Yuji IshikawaHymenolepisB.R. Mirdha and Shabeena RehmanMesocestoidesKerry A. Padgett, Paul R. Crosbie, and Walter M. BoyceSpirometraDongyou Liu, Jianshun Chen, and Weihuan FangTaeniaMunehiro Okamoto and Akira ItoSection IV Platyhelminthes: TrematodaClonorchisHeinz Mehlhorn, Boris Müller, and Jürgen SchmidtDicrocoeliumDonato Traversa, Vincenzo Lorusso, and Domenico OtrantoEchinostomesRafael Toledo and Bernard FriedFasciolaDongyou Liu and Xing-Quan ZhuFasciolopsisVeena Tandon, B. Roy, and P.K. PrasadGastrodiscoidesDongyou LiuHaplorchisUrusa Thaenkham and Jitra WaikagulHeterophyidaeRon Dzikowski and Michael G. LevyMetagonimusJae-Ran Yu and Jong-Yil ChaiNanophyetusDongyou LiuOpisthorchisPaiboon Sithithaworn, Thewarach Laha, and Ross H. AndrewsParagonimusHiromu Sugiyama, Takhellambam Shantikumar Singh, and Achariya RangsirujiPlagiorchisSun HuhSchistosoma and SchistosomiasisAntonio Muro and Luís Pérez del VillarTrichobilharziaP. Horák, F.M. Schets, L. Kolár?ová, and S.V. BrantSection V NematodaAncylostomaJaco J. VerweijAngiostrongylusDongyou LiuAnisakisStefano D’Amelio, Serena Cavallero, Marina Busi, Sofia Ingrosso, Elisabetta Giuffra, Sarra Farjallah, and Graça CostaAscarisDwight D. Bowman and Janice L. LiottaBaylisascarisDongyou LiuBrugiaKosum Chansiri and Supatra AreekitCapillariaDongyou LiuDioctophymeDongyou LiuDirofilariaAndrea Gustinelli, Maria Letizia Fioravanti, Monica Caffara, Lorella Garagnani, and Francesco RivasiDracunculusDongyou LiuEnterobiusDongyou LiuGnathostomaParon Dekumyoy, Tippayarat Yoonuan, and Jitra WaikagulHaemonchusMichael B. Hildreth and Aaron HarmonLoaFousseyni S. Touré NdouoMansonellaRamiro Morales-HojasMarshallagiaJesús M. Pérez and Luca RossiNecatorJan M. SchwenkenbecherOesophagostomumJaco J. VerweijOnchocercaRamiro Morales-HojasPseudoterranova and ContracaecumSimonetta Mattiucci, Michela Paoletti, Stephen C. Webb, and Giuseppe NascettiStrongyloidesDongyou LiuSyphaciaSanford H. Feldman and John H. PorterThelaziaDomenico Otranto and Donato TraversaToxocaraAnna BoreckaTrichinellaEdoardo Pozio and Giuseppe La RosaTrichostrongylusTai-Soon YongTrichurisC. Cutillas, M. de Rojas, and R. CallejónWuchereriaDongyou Liu and Frank W. AustinSection VI Arthropoda: ArachnidaArgasidae (Soft Ticks)Dongyou LiuDemodex (Hair Follicle Mite)Dongyou LiuDermanyssus, Ornithonyssus, and Trombicula (red, fowl, and harvest mites)Dongyou LiuIxodidae (Hard Ticks)Dongyou Liu and Chong YinPyroglyphidae and Glycyphagidae (House Dust and Storage Mites)Dongyou LiuSarcoptes (Itch Mite)Dongyou LiuSection VII Arthropoda: InsectaCalliphoridae, Oestridae, and Sarcophagidae (Myiasis-Causing Flies)Dongyou LiuCimex (Bedbug)Dongyou LiuPediculus (Body and Head Lice)Dongyou Liu and Sacha Stelzer-BraidPhthirus (Crab Louse)Dongyou LiuPulex, Xenopsylla, and Ctenocephalides (Human, Rat and Dog/Cat Fleas)Dongyou LiuTunga (Jigger Flea)Dongyou LiuSection VIII Arthropoda: PentastomidaArmillifer, Linguatula, and Porocephalus (Tongue Worms)Dongyou Liu