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galluzzi, lorenzo - molecular characterization of autophagic responses part a

Molecular Characterization of Autophagic Responses Part A




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Dettagli

Genere:Libro
Lingua: Inglese
Pubblicazione: 02/2017





Note Editore

Molecular Characterization of Autophagic Responses, Part A, presents a collection of methods for the qualitative and quantitative evaluation of virtually all the morphological, biochemical, and functional manifestations of autophagy, in vitro, ex vivo and in vivo, in organisms as distant as yeast and man.

Autophagy is an evolutionarily conserved mechanism for the lysosomal degradation of superfluous or dangerous cytoplasmic entities, and plays a critical role in the preservation of cellular and organismal homeostasis. Monitoring the biochemical processes that accompany autophagy is fundamental for understanding whether autophagic responses are efficient or dysfunctional.



  • Offers a detailed overview of the protocols used to study autophagy and various aspects of autophagic responses
  • Written in an accessible style by renowned experts in the field




Sommario

1. Correlative Live Cell and Super Resolution Imaging of Autophagosome Formation 2. Quantifying Autophagic Structures in Mammalian Cells Using Confocal Microscopy 3. The Use of DQ-BSA to Monitor the Turnover of Autophagy-Associated Cargo 4. Turnover of Lipidated LC3 and Autophagic Cargoes in Mammalian Cells 5. High-Throughput Quantification of GFP-LC3+ Dots by Automated Fluorescence Microscopy 6. Use of pHlurorin-mKate2-human LC3 to Monitor Autophagic Responses 7. Production of Human ATG Proteins for Lipidation Assays 8. Investigating Structure and Dynamics of Atg8 Family Proteins 9. Methods for Studying Interactions Between Atg8/LC3/GABARAP and LIR-Containing Proteins 10. Assessment of Posttranslational Modifications of ATG proteins 11. Tagged ATG8-Coding Constructs for the In Vitro and In Vivo Assessment of ATG4 Activity 12. Measurement of the Activity of the Atg4 Cysteine Proteases 13. Crystallographic Characterization of ATG Proteins and Their Interacting Partners 14. Dynamics of Atg5-Atg12-Atg16L1 Aggregation and Deaggregation 15. Fluorescent FYVE Chimeras to Quantify PtdIns3P Synthesis During Autophagy 16. Quantification of Phosphatidylinositol Phosphate Species in Purified Membranes 17. Mass Assays to Quantify Bioactive PtdIns3P and PtdIns5P During Autophagic Responses 18. Fluorescence-Based Assays to Analyse Phosphatidylinositol 5-Phosphate in Autophagy 19. Ultrastructural Characterization of Phagophores Using Electron Tomography on Cryoimmobilized and Freeze Substituted Samples 20. A Simple Cargo Sequestration Assay for Quantitative Measurement of Nonselective Autophagy in Cultured Cells 21. In Vitro Reconstitution of Autophagosome-Lysosome Fusion 22. In Vitro Reconstitution of Atg8 Conjugation and Deconjugation 23. Study of ULK1 Catalytic Activity and Its Regulation 24. Evaluating the mTOR Pathway in Physiological and Pharmacological Settings 25. Methods to Study the BECN1 Interactome in the Course of Autophagic Responses 26. In Vitro Characterization of VPS34 Lipid Kinase Inhibition by Small Molecules 27. Methods to Study Lysosomal AMPK Activation 28. Allosteric Modulation of AMPK Enzymatic Activity: In Vitro Characterization 29. Assessing the Catalytic Activity of Transglutaminases in the Context of Autophagic Responses





Autore

Lorenzo Galluzzi (born 1980) is currently Assistant Professor of Cell Biology in Radiation Oncology with the Department of Radiation Oncology of the Weill Cornell Medical College (New York, NY, USA), Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine (New Haven, CT, USA), Honorary Associate Professor with the Faculty of Medicine of the Paris Descartes University (Paris, France), and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara (Ferrara, Italy) and the Graduate School of Pharmacological Sciences of the University of Padova (Padova, Italy).

Prior to joining Weill Cornell Medical College (2017), Lorenzo Galluzzi was a Junior Scientist of the Research Team "Apoptosis, Cancer and Immunity at the Cordeliers Research Center (Paris, France; 2012-2016). Lorenzo Galluzzi did his post-doctoral training at the Gustave Roussy Cancer Center (Villejuif, France; 2009-2011), after receiving his PhD from the Paris Sud University (Le Kremlin-Bicetre, France; 2008). He is also Associate Director of the European Academy for Tumor Immunology (EATI), Co-chair of the Society for Immunotherapy of Cancer (SITC) Immunogenic Cell Death Working Group, and Founding Member of the European Research Institute for Integrated Cellular Pathology (ERI-ICP).

Lorenzo Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. In particular, he provided profound insights into the links between adaptive stress responses in cancer cells and the activation of a clinically relevant tumor-targeting immune response in the context of chemotherapy and radiation therapy. Lorenzo Galluzzi has published more than 400 scientific articles in international peer-reviewed journals. According to a survey published by Lab Times, he is currently the 6th and the youngest of the 30 most-cited European cell biologists (relative to the period 2007-2013), and he was nominated Highly Cited Researcher by Clarivate Analytics (formerly, Thomson Reuter) in 2016 and 2018.

Lorenzo Galluzzi currently operates as Editor-in-Chief of three journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, and Molecular and Cellular Oncology (which he co-founded in 2013). In addition, Lorenzo Galluzzi currently serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease.
Lorenzo Galluzzi (born 1980) is currently Assistant Professor of Cell Biology in Radiation Oncology with the Department of Radiation Oncology of the Weill Cornell Medical College (New York, NY, USA), Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine (New Haven, CT, USA), Honorary Associate Professor with the Faculty of Medicine of the Paris Descartes University (Paris, France), and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara (Ferrara, Italy) and the Graduate School of Pharmacological Sciences of the University of Padova (Padova, Italy).

Prior to joining Weill Cornell Medical College (2017), Lorenzo Galluzzi was a Junior Scientist of the Research Team "Apoptosis, Cancer and Immunity at the Cordeliers Research Center (Paris, France; 2012-2016). Lorenzo Galluzzi did his post-doctoral training at the Gustave Roussy Cancer Center (Villejuif, France; 2009-2011), after receiving his PhD from the Paris Sud University (Le Kremlin-Bicetre, France; 2008). He is also Associate Director of the European Academy for Tumor Immunology (EATI), Co-chair of the Society for Immunotherapy of Cancer (SITC) Immunogenic Cell Death Working Group, and Founding Member of the European Research Institute for Integrated Cellular Pathology (ERI-ICP).

Lorenzo Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. In particular, he provided profound insights into the links between adaptive stress responses in cancer cells and the activation of a clinically relevant tumor-targeting immune response in the context of chemotherapy and radiation therapy. Lorenzo Galluzzi has published more than 400 scientific articles in international peer-reviewed journals. According to a survey published by Lab Times, he is currently the 6th and the youngest of the 30 most-cited European cell biologists (relative to the period 2007-2013), and he was nominated Highly Cited Researcher by Clarivate Analytics (formerly, Thomson Reuter) in 2016 and 2018.

Lorenzo Galluzzi currently operates as Editor-in-Chief of three journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, and Molecular and Cellular Oncology (which he co-founded in 2013). In addition, Lorenzo Galluzzi










Altre Informazioni

ISBN:

9780128096758

Condizione: Nuovo
Collana: Methods in Enzymology
Dimensioni: 229 x 152 mm
Formato: Copertina rigida
Pagine Arabe: 598


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