miRNA Biogenesis Methods and Protocols

This detailed volume collects chapters that seek to expand our knowledge of molecular events and extended molecular networks in gene regulation. The contents explore numerous aspects of miRNA biogenesis, a sophisticated series of events that assure a finely tuned regulation of miRNA expression and activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, miRNA Biogenesis: Methods and Protocols aims to contribute to the further development of knowledge and application of miRNA biogenesis, both in the basic research lab and in the clinic. 

Chapter 1       microRNA biogenesis Review

Chapter 2       Identifying microRNA transcription sites

Chapter 3       Annotating primary microRNAs

Chapter 4       Defining Microprocessor co-factors

Chapter 5       Endogenous assay for primary microRNA processing

Chapter 6       In vitro assay for primary microRNA processing

Chapter 7       miRtrons

Chapter 8       Inhibiting primary microRNA processing

Chapter 9       Precursor microRNA characterization

Chapter 10     Precursor microRNA export

Chapter 11     Processing of precursor microRNAs in the cytoplasm

Chapter 12     Composition of the RNA-induced Silencing Complex

Chapter 13     RNA-sequencing of mature miRNAs

Chapter 14     qPCR measurement of mature microRNAs

Chapter 15     Stability of mature microRNAs

Chapter 16     MicroRNA modifications

Chapter 18     RNA modifications in microRNA biogenesis

Chapter 19     MicroRNA biogenesis in disease Case study