Microbial Toxins - Holst Otto (Curatore) | Libro Humana 05/2011 - HOEPLI.it

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Microbial Toxins Methods and Protocols

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Lingua: Inglese


Pubblicazione: 05/2011
Edizione: 2011


Interest in the field of microbial toxins is ever growing and spreading across a broad spectrum of scientific disciplines. In an effort to supplement the available reference texts on toxins, Microbial Toxins: Methods and Protocols includes protocols on mold fungus toxins, with some focus on aflatoxins. Intended to support a wide variety of researchers, Microbial Toxins: Methods and Protocols presents the reader with biological, chemical, physical, and medical approaches, as well as state-of-the-art research techniques. Divided into three convenient sections, this detailed volume covers bacterial protein toxins, endotoxins, and mold fungus toxins. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Microbial Toxins: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential field.


Part I Bacterial Protein Toxins


1.   Detection of Bacterial Protein Toxins by Solid Phase Magnetic Immunocapture and Mass Spectrometry

            Gabriella Pocsfalvi and Gitta Schlosser


2.   Sensitive and Rapid Detection of Cholera Toxin-Producing Vibrio cholerae using Loop-Mediated Isothermal Amplification

                  Wataru Yamazaki


3.   Ultra Sensitive Detection of Botulinum Neurotoxins and Anthrax Lethal Factor in Biological Samples by ALISSA

                  Karine Bagramyan and Markus Kalkum


4.   Examination of Bacillus anthracis Spores by Multiparameter Flow Cytometry

                  William C. Schumacher, Craig A. Storozuk, Prabir K. Dutta, and Andrew J. Phipps


5.   A Cell-Based Fluorescent Assay to Detect the Activity of Shiga Toxin and Other Toxins That Inhibit Protein Synthesis

                  Shane Massey, Beatriz Quiñones, and Ken Teter


6.   Use of a Vero Cell-Based Fluorescent Assay to Assess Relative Toxicities of Shiga Toxin 2 Subtypes from Escherichia coli

                  Beatriz Quiñones and Michelle S. Swimley


7.   Molecular Methods – Chip Assay and Quantitative Real-Time PCR – in Detecting Hepatotoxic Cyanobacteria

                  Anne Rantala-Ylinen, Hanna Sipari, and Kaarina Sivonen



Part II Endotoxins


8.   Capillary Electrophoresis Chips for Fingerprinting Endotoxin Chemotypes from Whole-Cell Lysates

                  Béla Kocsis, Anikó Kilár, Lilla Makszin, Krisztina Kovács, and Ferenc Kilár


9.   Isolation of Smooth-Type Lipopolysaccharides to Electrophoretic Homogeneity

                  Elder Pupo


10. A Method for Unobtrusive Labeling of Lipopolysaccharides with Quantum Dots

                  Carlos Morales-Betanzos, Maria Gonzalez-Moa, and Sergei A. Svarovsky


11. Fluorescence-Based Methods to Assay Inhibitors of Lipopolysaccharide Synthesis

                  Marcy Hernick


12. Micromethods for Lipid A Isolation and Structural Characterization

                  Martine Caroff and Alexey Novikov


13. Two Efficient Methods for the Conjugation of Smooth-Form Lipopolysaccharides (LPS) with Probes Bearing Hydrazine or Amino Groups. I. LPS Activation with Cyanogen Bromide

                  Fernando Battaglini and Diego Pallarola


14. Two Efficient Methods for the Conjugation of Smooth-Form Lipopolysaccharides (LPS) with Probes Bearing Hydrazine or Amino Groups. II. LPS Activation with a Cyanopyridinium Agent

                  Fernando Battaglini and Diego Pallarola



Part III Mold Fungus Toxins


15. Extraction and Analysis of Fumonisins and Compounds Indicative of Fumonisin Exposure in Plant and Mammalian Tissues and Cultured Cells

                  Nicholas C. Zitomer and Ronald T. Riley


16. Determination of Fumonisins B1 and B2 in Maize Food Products by a New Analytical Method Based on High-Performance Liquid Chromatography and Fluorometric Detection with Post-Column Derivatization

            Marilena Muscarella, Sonia Lo Magro, Donatella Nardiello, Carmen Palermo, and Diego Centonze


17. A Confirmatory Method for Aflatoxin M1 Determination in Milk Based on Immunoaffinity Clean-Up and High-Performance Liquid Chromatography with Fluorometric Detection

            Marilena Muscarella, Sonia Lo Magro, Carmen Palermo, and Diego Centonze

18. Simultanious Determination of Aflatoxins B1, B2, G1 and G2 in Foods and Feed Materials by High-Performance Liquid Chromatography with On-Line Photochemical Derivatization and Fluorescence Detection

            Marilena Muscarella, Marco Iammarino, Donatella Nardiello, Sonia Lo Magro, Carmen Palermo, and Diego Centonze


19. Highly Sensitive PCR-Based Detection Specific to Aspergillus flavus

                  Amaia González-Salgado, Teresa González-Jaén, Covadonga Vázquez, and Belén Patiño


20. A Rapid Enzymatic Method for Aflatoxin B Detection

                  Danila Moscone, Fabiana Arduini, and Aziz Amine

Altre Informazioni



Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 254 x 178 mm Ø 678 gr
Formato: Copertina rigida
Pagine Arabe: 240
Pagine Romane: xii

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