Microbial Toxins Methods and Protocols

This second edition volume expands on the previous editions with detailed protocols on (cyano) bacterial and mold fungus toxins, and a specific focus on Gram-negative endotoxins (lipopolysaccharides). The chapters in Microbial Toxins: Methods and Protocols, Second Edition present techniques that cover topics such as detection of cholera toxin by an immunochromatographic test strip; label-free immune-sensors for the fast detection of Listeria in food; endotoxin removal from Escherichia coli bacterial lysate using biphasic liquid system; and micromethods for isolation and structural characterization of lipid A, and polysaccharide regions of bacterial lipopolysaccharides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Microbial Toxins: Methods and Protocols, Second Edition is a valuable and useful resource for scientists who are interested in this field, and is a great tool for researchers who are looking to learn about new, particular techniques to further enhance their work.

Preface...

Table of Contents...

Contributing Authors....

1. Detection of Cholera Toxin by an Immunochromatographic Test Strip
Eiki Yamasaki, Ryuta Sakamoto, Takashi Matsumoto, Biswajit Maiti, Kayo Okumura, Fumiki Morimatsu, G. Balakrish Nair, and Hisao Kurazono

 2. Electrochemical Aptamer Scaffold Biosensors for Detection of Botulism and Ricin Proteins
Jessica Daniel, Lisa Fetter, Susan Jett, Teisha J. Rowland, and Andrew J. Bonham

 3. A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis
Patrick Cherubin, Beatriz Quiñones, Salem Elkahoui, Wallace Yokohama, and Ken Teter

4. Molecular Methods for Identification of Clostridium tetani by Targeting Neurotoxin
Basavraj Nagoba, Mahesh Dharne, and Kushal N. Gohil

5.  Label-Free Immuno-Sensors for the Fast Detection of Listeria in Food
Alexandra Morlay, Agnès Roux, Vincent Templier, Félix Piat, and Yoann Roupioz

6.  Aptamer-Based Trapping: Enrichment of Bacillus cereus Spores for Real-Time PCR Detection
Christin Fischer and Markus Fischer

8.  A Method to Prepare Magnetic Nanosilicate Platelets for Effective Removal of Microcystis aeruginosa and Microcystin LR
Shu-Chi Chang, Bo-Li Lu, Jiang-Jen Lin, Yen-Hsien Li, and Maw-Rong Lee

9.  An Immunochromatographic Test Strip to Detect Ochratoxin A and Zearalenone Simultaniously
Xiaofei Hu and Gaiping Zhang

10.  Endotoxin Removal from Escherichia coli Bacterial Lysate using Biphasic Liquid System
Janusz Boratynski and Bozena Szermer-Olearnik

11.  Fourier Transform Infrared Spectroscopy as a Tool in Analysis of Proteus mirabilis Endotoxins
Paulina Zarnowiec, Grzegorz Czerwonka, and Wieslaw Kaca

12.  Laser Interferometry Method as a Novel Tool in Endotoxin Research
Michal Arabski and Slawomir Wasik

13.  Endotoxin Entrapment on Glass via C18 Self-Assembled Monolayers and Rapid Detection using Drug-Nanoparticle Bioconjugate Probes
Prasanta Kalita, Anshuman Dasgupta, and Shalini Gupta

14.  A Bioassay for Determination of Lipopolysaccharides and Lipoproteins
Marcus Peters, Petra Bonowitz, and Albrecht Bufe

17.  Mass Spectrometry for Profiling LOS and Lipid A Structures from Whole-Cell Lysates: Directly from a Few Bacterial Colonies or from Liquid Broth Cultures
Béla Kocsis, Anikó Kilár, Szandra Péter, Ágnes Dörnyei, Viktor Sándor, and Ferenc Kilár