Microarray Technology Through Applications - Falciani Francesco (Curatore) | Libro Taylor & Francis 03/2007 - HOEPLI.it


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falciani francesco (curatore) - microarray technology through applications

Microarray Technology Through Applications




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Dettagli

Genere:Libro
Lingua: Inglese
Pubblicazione: 03/2007
Edizione: 1° edizione





Note Editore

Microarray Technology Through Applications provides the reader with an understanding, from an applications perspective, of the diverse range of concepts required to master the experimental and data analysis aspects of microarray technology. The first chapteris a concise introduction to the technology and provides the theoretical background required to understand the subsequent sections.The followingchaptersare a series ofcase studies representative of the most general and important applications of microarray technology, including CGH, analysis of gene expression, SNP arrays and protein arrays. The case studiesare written by experts in the field and describe prototypic projects, indicating how to generalize the approach to similar studies.There are detailed step-by-step protocols describing the specific experimental and data analysis protocols mentioned in the case study section. Thereis alsoinformation on printing glass DNA microarray slides and data interpretation. Colour figures and data sets are provided on the website at http://www.garlandscience.com/9780415378536




Sommario

1. Introduction to Microarray Technology 2. Immunoprecipitation with Microarrays to Determine the Genome-Wide Binding Profile of a DNA-Associated Protein 3. Array-Based Comparative Genomic Hybridization (Array CGH) as a Tool for Solving Practical Biological and Medical Questions 4. Use of SNPs Arrays : Design, Tools and Applications 5. In vitro Analysis of Gene Expression 6. The Analysis of Cellular Transcriptional Response at the Genome Level : Two Case Studies with Relevance to Bacterial Pathogenesis 7. Functional Annotation of Microarray Experiments 8. Microarray Technology in Agricultural Research 9. Protein Microarrays Protocol 1. Microarray Printing - Standard Operating Procedure for QIAGEN OPERON Oligonucleotide Arrays Protocol 2. E. coli RNA Extraction Protocol 3. Indirect Labeling of First-strand cDNA Probes Protocol 4. Pre-Hybridization and Washing Protocol for UltraGAP Slides Protocol 5. ChIP Procedure Protocol 6. Array CGH Protocol 7. In vitro Analysis of Gene Expression Using Run-off Microarray Analysis of Gene Expression (ROMA) Appendix 1. Notes on Printing Glass DNA Microarray Slides Appendix 2. Comparative Genomics : The Nature of CGH Analysis and Data Interpretation Appendix 3. Useful Links Appendix 4. Table 7




Autore

Francesco Falciani, Senior Lecturer in Bioinformatics, School of Biosciences, University of Birmingham, UK







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Altre Informazioni

ISBN:

9780415378536

Condizione: Nuovo
Dimensioni: 9.75 x 6.75 in Ø 1.35 lb
Formato: Brossura
Illustration Notes:58 b/w images, 13 tables, 6 halftones, 18 color halftones and 34 line drawings
Pagine Arabe: 276






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